Merck
All Photos(1)

4352157

Sigma-Aldrich

Trypsin, TPCK-Treated

All Photos(1)
Synonym(s):
6- (1-tosylamido-2-phenyl) ethyl chloromethyl ketone treated Trypsin, Modified trypsin (TPCK-Treated), Trypsin, TPCK treated
NACRES:
NA.56

Recommended Products

Slide 1 of 10

1 of 10

Sigma-Aldrich

Sigma-Aldrich

4370285

Trypsin, TPCK-Treated

Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Sigma-Aldrich

T1426

胰蛋白酶 来源于牛胰腺

TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Trypsin from bovine pancreas TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Sigma-Aldrich

T8802

胰蛋白酶 来源于牛胰腺

TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein

Trypsin from porcine pancreas lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

Sigma-Aldrich

T4799

胰蛋白酶 来源于猪胰腺

lyophilized powder, BioReagent, suitable for cell culture, 1,000-2,000 BAEE units/mg solid

Trypsin Acetylated from bovine pancreas Type V-S, ≥8,500 BAEE units/mg protein (biuret)

Sigma-Aldrich

T6763

乙酰化胰蛋白酶 来源于牛胰腺

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

Sigma-Aldrich

Sigma-Aldrich

T6567

胰蛋白酶 来源于猪胰腺

Proteomics Grade, BioReagent, Dimethylated

Trypsin from bovine pancreas suitable for protein sequencing, lyophilized powder

Sigma-Aldrich

T8658

胰蛋白酶 来源于牛胰腺

suitable for protein sequencing, lyophilized powder

Sigma-Aldrich

Sigma-Aldrich

6502

Trypsin, Bovine Pancreas

vibrant-m

T2600000

胰蛋白酶

BRP, European Pharmacopoeia (EP) Reference Standard

α-Chymotrypsin from bovine pancreas Type II, lyophilized powder, ≥40 units/mg protein

Sigma-Aldrich

C4129

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

运输

dry ice

储存温度

−20°C

Related Categories

一般描述

Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of Lysine and Arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. This trypsin product also contains calcium chloride, which may improve consistency in digestion. Each package contains 10 vials, with 25 μg in each vial. 

应用

Trypsin, TPCK-Treated has been used for the digestion of protein samples to generate peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.
Trypsin, TPCK-Treated has been used in enzymatic treatment to cleave the recombinant native B-cell lymphoma-extra large (Bcl-xL)-ΔTM monomer to generate fibrils (the third type of Bcl-xL fibril, called “BclxLcf37”). It has also been used in influenza infection experiments as a supplement in infection/DMEM serum-free medium to infect A549 cells with influenza A H1N1 virus at a multiplicity of infection (MOI) to perform multi-round infection.

包装

1 kit

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H315 - H319 - H334

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service