Merck
所有图片(1)

4370285

Sigma-Aldrich

Trypsin, TPCK-Treated

所有图片(1)
别名:
Anti-HEL114
EC 号:
232-650-8
NACRES:
NA.56

运输

dry ice

储存温度

−20°C

一般描述

胰蛋白酶是一种丝氨酸蛋白酶，可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联苯氯甲基酮 (TPCK) 处理，可使外源性糜蛋白酶失活。每个包装含8个小瓶，每瓶含25 μg。

应用

TPCK处理的胰蛋白酶已被用于:
  • 作为杜尔贝科改良伊格尔培养基（DMEM）的补充剂，用于上皮样猪肾细胞系（LLC-PK1） 的猪Delta冠状病毒（PDCoV）感染实验
  • 分离人脐静脉内皮细胞（HUVEC） 用于膜联蛋白-V/碘化丙啶 (PI) 染色测定
  • 用于多循环复制动力学的最小必需培养基 （MEM）

生化/生理作用

N-对-对甲苯磺酰基-L-苯丙氨酸氯甲基酮（TPCK） 可用作糜蛋白酶的不可逆抑制剂。胰蛋白酶诱导人纤维细胞分化。胰蛋白酶被广泛用于蛋白质组学中的蛋白质样品消化。在细胞培养中，进行胰蛋白酶消化是为了使贴壁细胞彼此分开并离开培养容器壁。

象形图

Exclamation markHealth hazard
GHS07,GHS08

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

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Michael J V White et al.
PloS one, 8(8), e70795-e70795 (2013-08-21)
Trypsin-containing topical treatments can be used to speed wound healing, although the mechanism of action is unknown. To help form granulation tissue and heal wounds, monocytes leave the circulation, enter the wound tissue, and differentiate into fibroblast-like cells called fibrocytes.
Zdeněk Perutka et al.
Molecules (Basel, Switzerland), 23(10) (2018-10-17)
Trypsin is the protease of choice for protein sample digestion in proteomics. The most typical active forms are the single-chain β-trypsin and the two-chain α-trypsin, which is produced by a limited autolysis of β-trypsin. An additional intra-chain split leads to
Zhonghui Ling et al.
Reproductive biology, 21(2), 100483-100483 (2021-02-26)
Vascular endothelial cell damage is regarded as the carrier in the progression of the pathological changes of preeclampsia (PE) from the placenta to maternal organs. MicroRNA (miR)-141-3p was aberrantly expressed during PE pathogenesis. We investigated the role of miR-141-3p in
Yu Bai et al.
Microbial pathogenesis, 150, 104645-104645 (2020-12-08)
Influenza virus is responsible for significant morbidity and mortality worldwide. Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is the major cause of death in influenza virus infected patients. Recent studies indicated that active glucagon like peptide-1 (GLP-1) encoded by
Jian-Ping Dai et al.
International journal of molecular sciences, 19(4) (2018-03-24)
Oxymatrine (OMT) is a strong immunosuppressive agent that has been used in the clinic for many years. In the present study, by using plaque inhibition, luciferase reporter plasmids, qRT-PCR, western blotting, and ELISA assays, we have investigated the effect and
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