Merck
All Photos(1)

4370285

Sigma-Aldrich

Trypsin, TPCK-Treated

Synonym(s):
Trypsin, TPCK treated for Cell Culture
EC 号:
NACRES:
NA.56

运输

dry ice

储存温度

−20°C

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43521571.0844410109878001
Sigma-Aldrich

Sigma-Aldrich

1.08444

Trypsin

Trypsin Inhibitor from chicken egg white

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10109878001

胰蛋白酶抑制剂

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

一般描述

胰蛋白酶是一种丝氨酸蛋白酶,可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联苯氯甲基酮 (TPCK) 处理,可使外源性糜蛋白酶失活。每个包装含8个小瓶,每瓶含25 μg。

应用

TPCK处理的胰蛋白酶已被用于:
  • 作为杜尔贝科改良伊格尔培养基(DMEM)的补充剂,用于上皮样猪肾细胞系(LLC-PK1) 的猪Delta冠状病毒(PDCoV)感染实验
  • 分离人脐静脉内皮细胞(HUVEC) 用于膜联蛋白-V/碘化丙啶 (PI) 染色测定
  • 用于多循环复制动力学的最小必需培养基 (MEM)

生化/生理作用

N--对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK) 可用作糜蛋白酶的不可逆抑制剂。胰蛋白酶诱导人纤维细胞分化。胰蛋白酶被广泛用于蛋白质组学中的蛋白质样品消化。在细胞培养中,进行胰蛋白酶消化是为了使贴壁细胞彼此分开并离开培养容器壁。

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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