46950

荧光素 5(6)-异硫氰酸酯

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

• Synonym(s): FITC, 异硫氰酸荧光素
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 27072-45-3
• Molecular Weight: 389.38
• Beilstein: 1407295
• EC 号: 248-207-7
• MDL编号: MFCD00005063
• PubChem化学物质编号: 329757273
• NACRES: NA.32

Properties

• 质量水平: 100
• 产品线: BioReagent
• 测定: ≥90% (HPLC)
• 荧光: λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
• 适用性: suitable for fluorescence
• 储存温度: −20°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

一般描述

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

应用

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.

用于蛋白质的荧光标记（例如免疫荧光）;凝胶色谱中的 H 蛋白质标记

包装

无底玻璃瓶。内容物在插入的熔锥内。

Safety Information

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 个人防护装备: dust mask type N95 (US), Eyeshields, Faceshields, Gloves