Merck
All Photos(1)

51412C

SAFC

最低必需培养基

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
Eagle最低必需培养基, MEM
NACRES:
NA.75

描述

for research or for further manufacturing use

质量水平

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

组分

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

储存温度

2-8°C

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最低必需培养基

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sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

form

liquid

form

liquid

form

dry powder

form

liquid

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

components

Earle’s salts (5% CO2): yes
L-glutamine: no

components

phenol red: 10.620 mg/L
L-glutamine: 292 mg/L
NaHCO3: no
Earle’s salts (5% CO2): yes

components

Earle’s salts (5% CO2): yes
phenol red: 10.62 mg/L
NaHCO3: 2200 mg/L
L-glutamine: 290 mg/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

400

Quality Level

500

Quality Level

500

一般描述

Harry Eagle研究了小鼠L细胞和HeLa细胞在培养中的基本营养需求,因此开发了含有Earle平衡盐的最低必需培养基(MEM)。他证明这些细胞需要13种氨基酸和7种维生素才能在体外生长和繁殖。MEM是Eagle早期培养基基础培养基Eagle(BME)的改良,含有更高浓度的必需营养素。这些培养基已证明能够在培养中支持多种正常细胞和转化细胞,并含有Earle平衡盐,这使其适合在含有CO2的大气中使用。

应用

最小必需培养基 (MEM) 已被用作骨髓间充质干细胞 (BMSCs) 和神经元的生长培养基。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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