Merck

88021102

Chang Liver (HeLa derivative) cell line

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别名:
Chang Liver cell line (HeLa derivative)

生物来源

human cervix

质量水平

形式

liquid

生长模式

Adherent

核型

Modal no. 70

形态学

Epithelial

产品

Not specified

受体

Not specified

technique(s)

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

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INT 407 (HeLa derivative) cell line from human cervix

85051004

INT 407 (HeLa derivative) cell line

growth mode

Adherent

growth mode

Adherent

growth mode

-

growth mode

-

karyotype

Modal no. 70

karyotype

2n = 46, HeLa markers

karyotype

-

karyotype

-

morphology

Epithelial

morphology

Epithelial

morphology

-

morphology

-

products

Not specified

products

Not specified

products

-

products

-

receptors

Not specified

receptors

Not specified

receptors

-

receptors

-

细胞系来源

Human cervix carcinoma

细胞系描述

The cell line Chang Liver was originally thought to be derived from normal liver. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

应用

Chang Liver (HeLa derivative) cell line has been used to study the construction and biological activity of human torque teno virus (TTV) genotype 6.
Virus, biochemical and replication studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,14
D16S539: 9,10
D5S818: 12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

免责声明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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Clonal variation and interaction of cells with viruses.
W H MURPHY et al.
National Cancer Institute monograph, 7, 249-271 (1962-04-01)
Kofi D Kwofie et al.
Antimicrobial agents and chemotherapy, 60(6), 3283-3290 (2016-03-10)
Trypanosoma brucei parasites are kinetoplastid protozoa that devastate the health and economic well-being of millions of people in Africa through the disease human African trypanosomiasis (HAT). New chemotherapy has been eagerly awaited due to severe side effects and the drug
Construction and biological activity of a full?length molecular clone of human Torque teno virus (TTV) genotype 6
Kakkola L, et al.
FEBS Journal, 274(18), 4719-4730 (2007)
Continuous subcultivation of epithelial-like cells from normal human tissues.
R S M CHANG
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 87(2), 440-443 (1954-11-01)
Examination of ATCC stocks for HeLa marker chromosomes in human cell lines.
K S Lavappa et al.
Nature, 259(5540), 211-213 (1976-01-22)

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