94403

Hoechst 33258 溶液

1 mg/mL in H₂O, ≥98.0% (HPLC)

• Synonym(s): 赫斯特荧光染料 33258 溶液
• Empirical Formula (Hill Notation): C₂₅H₂₄N₆O · 3HCl
• CAS Number: 23491-45-4
• Molecular Weight: 533.88
• PubChem化学物质编号: 329770334
• NACRES: NA.32

Properties

• 质量水平: 100
• 测定: ≥98.0% (HPLC)
• 形式: liquid
• 浓度: 1 mg/mL in H₂O
• 溶解性: DMF: soluble; H₂O: soluble
• 荧光: λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• 储存温度: 2-8°C
• SMILES string: Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
• InChI key: SMNPLAKEGAEPJD-UHFFFAOYSA-N

Description

一般描述

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

应用

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

Safety Information

• 储存分类代码: 12 - Non Combustible Liquids
• 闪点(F): Not applicable
• 闪点(C): Not applicable