Merck

A0168

Sigma-Aldrich

抗-小鼠IgG(Fc特异性)-过氧化物酶 山羊抗

affinity isolated antibody

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别名:
Goat Anti-Mouse IgG (Fc specific)–HRP
MDL编号:
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

peroxidase conjugate

抗体形式

affinity isolated antibody

antibody product type

secondary antibodies

克隆

polyclonal

species reactivity

mouse

technique(s)

direct ELISA: 1:50,000
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
A9309A3682A2304
conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, immunocytochemistry: suitable, western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section, western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells

biological source

goat

biological source

goat

biological source

goat

biological source

goat

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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一般描述

免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。
通过免疫特异性纯化从山羊抗小鼠IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与小鼠IgG的Fc片段特异性结合)。以0.2%戊二醛为交联剂,将抗鼠IgG与过氧化物酶偶联。该抗体制剂采用人IgG吸附固相制备,以确保组织或细胞制剂中的交叉反应性最低。

免疫原

纯化的小鼠IgG Fc片段

应用

山羊抗小鼠IgG (Fc特定)-过氧化物酶抗体用于:
  • 免迹印迹
  • 酶联免疫吸附测定(ELISA)和
  • 间接免疫过氧化物酶测定(IIPA)

通过使用生物合成PND和链霉亲和素涂层板进行ELISA试验,从而对免疫小鼠血液中一种新的PND抗体的特异性进行检测。HRP缀合的山羊抗小鼠IgG(Fc特异性)可用作二抗。

外形

0.01M磷酸缓冲盐缓冲液(pH7.4, 含有0.05% MIT)溶液。

制备说明

通过Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978)等描述的两步戊二醛方法进行制备。

免责声明

除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Human antibodies targeting CD30+ lymphomas
Wezler X, et al.
Human Antibodies, 21(1-2), 13-28 (2012)
Intrathecal synthesis of specific antibodies in patients with invasion of the central nervous system by Mycoplasma pneumoniae.
Benvcina D, et al.
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 19(7), 521-530 (2000)
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Human Vaccines & Immunotherapeutics, 13(5), 1094-1104 (2017)
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Human vaccines & immunotherapeutics, 10(7), 2074-2080 (2014-11-27)
In recipients primed with acellular pertussis diphtheria-tetanus combined vaccine (DTaP) an increased incidence of severe local reactions with extensive redness/swelling has been reported for each subsequent dose of diphtheria-tetanus based combination vaccine given as a booster. This has been attributed
Anti-complement component 5 antibody targeting MG4 domain inhibits choroidal neovascularization
Jo DH, et al.
Testing, 8(28), 45506-45506 (2017)

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