A2066

Sigma-Aldrich

抗肌动蛋白抗体 兔抗

affinity isolated antibody, buffered aqueous solution

别名:
Sigma抗肌动蛋白抗体, 用于蛋白质免疫印迹分析的抗肌动蛋白抗体 - 兔抗肌动蛋白抗体, 抗肌动蛋白, 用于蛋白质免疫印迹分析的抗肌动蛋白抗体, Sigma肌动蛋白抗体
MDL编号:
NACRES:
NA.41

生物来源

rabbit

质量水平

200

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

分子量

antigen 42 kDa

species reactivity

wide range, human, chicken, amoeba, slime mold, vertebrates

应用

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections
indirect immunofluorescence: 1:40 using chicken fibroblasts
western blot: 1:100 using chicken gizzard extract

偶联物

unconjugated

特色行业

Research Pathology

UniProt登记号

运输

dry ice

储存温度

−20°C

Gene Information

human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)

一般描述

肌动蛋白是一种高度保守的蛋白质,是所有细胞类型中细胞骨架和收缩结构的主要成分。肌动蛋白含量与分化类型以及细胞和组织的功能状态有关。肌动蛋白具有两种不同的聚集形式,即球状或纤维状,并且在脊椎动物中至少具有六种不同亚型。肌动蛋白表现出大于90%的序列同源性,但每种亚型都具有不同的NH 2-末端序列。
ACTA1(肌动蛋白,α 1)基因位于人染色体1q42.13上。该基因编码肌动蛋白1,它是骨骼肌的细丝成分。

在免疫印迹中,本品可用于定位许多物种的肌动蛋白,范围从人骨骼肌到变形虫。在免疫印迹分析中,本品可识别人或动物组织提取物中的42kDa肌动蛋白条带。

特异性

抗肌动蛋白抗体可以对鸡成纤维细胞培养物中的典型张力纤维进行特异性染色。

免疫原

与多抗原肽(MAP)骨架连接的C末端肌动蛋白片段。

应用

可用于研究肌动蛋白结构和功能,并检测肌动蛋白结合蛋白的结合位点。
兔抗肌动蛋白抗体可用于蛋白质免疫印迹分析。该抗体还可用于免疫组织化学(1:25,使用人或动物组织切片)和间接免疫荧光(1:40,使用鸡成纤维细胞)应用。
用于研究肌动蛋白结构和功能,并检测肌动蛋白结合蛋白的结合位点。使用肌动蛋白作为上样对照品,通过蛋白质免疫印迹法分析从胚胎小鼠肢体组织产生的蛋白质裂解物。使用以1:8000比例稀释的兔抗肌动蛋白抗体检测肌动蛋白。将兔抗肌动蛋白抗体检测的肌动蛋白用作MCF7细胞裂解物的上样对照。在42 kDa处检测到肌动蛋白。
用于研究肌动蛋白结构和功能,并检测肌动蛋白结合蛋白的结合位点。使用肌动蛋白作为上样对照品,通过蛋白质免疫印迹法分析从胚胎小鼠肢体组织产生的蛋白质裂解物。使用以1:8000比例稀释的兔抗肌动蛋白抗体检测肌动蛋白。将兔抗肌动蛋白抗体检测的肌动蛋白用作MCF7细胞裂解物的上样对照。在42 kDa处检测到肌动蛋白。

生化/生理作用

肌动蛋白是一种细胞骨架蛋白,可调节细胞运动、分泌、吞噬作用和胞质分裂。肌动蛋白的NH2末端具有抗原的作用。该末端还可以调节肌动蛋白相互作用,并且可以与肌球蛋白等蛋白质结合。肌动蛋白对于成熟血小板的形成至关重要。
已知ACTA1(肌动蛋白,α 1)突变可导致细肌丝肌病,如线状体肌病、先天性肌病、肌动蛋白肌病、核内棒状体肌病、帽状肌病和轴空性肌病。在没有任何早期自身免疫性肝病证据的情况下,在原位肝移植中已观察到该基因的上调。编码蛋白质肌动蛋白形成细丝网络并调控细胞形状。它提供细胞强度并促进细胞的主动运动。

外形

溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有1%牛血清白蛋白以及15 mM 叠氮化钠作为防腐剂。

储存及稳定性

若需连续使用,可在2-8°C下保存最长一个月。
若需延长储存时间,可将溶液分装并冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊,请在使用前通过离心澄清溶液。

其他说明

想要查看肌动蛋白抗体选择指南,请访问 www.sigmaaldrich.com/actin

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. What is the molecular weight of actin, Products  A2066 and A2547?

    Actin has a molecular weight of 42 kDa.

  3. Which type of actin does Product A2066, Anti-Actin antibody produced in rabbit, recognize?

    This antibody is a polyclonal antibody.  The immunogen was a C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) that is found in all actin sequences.

  4. Does Product A2066, Anti-Actin antibody produced in rabbit, react with my species?

    The sequence use to develop this antibody is conserved across many species from amoeba to vertebrates.  It should react with most samples.

  5. Are there any special considerations for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections?

    For FFPE sections, we recommend using enzymatic unmasking.

  6. Why do I have 3 bands on my western blot when using Product A2066, Anti-Actin antibody produced in rabbit?

    While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well.  Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93, 2234-2238 (1996).

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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技术文章
Loading controls in western blotting application.
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