affinity isolated antibody
buffered aqueous solution
antigen 42 kDa
wide range, human, chicken, amoeba, slime mold, vertebrates
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25 using human or animal tissue sections
indirect immunofluorescence: 1:40 using chicken fibroblasts
western blot: 1:100 using chicken gizzard extract
NONH for all modes of transport
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Actin has a molecular weight of 42 kDa.
This antibody is a polyclonal antibody. The immunogen was a C-terminal actin fragment (C11 peptide, Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe) that is found in all actin sequences.
The sequence use to develop this antibody is conserved across many species from amoeba to vertebrates. It should react with most samples.
For FFPE sections, we recommend using enzymatic unmasking.
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93, 2234-2238 (1996).
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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