Merck
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A2153

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

lyophilized powder, ≥96% (agarose gel electrophoresis)

别名:
BSA, 牛血清白蛋白
CAS号:
EC 号:
MDL编号:
NACRES:
NA.27

质量水平

200

生物来源

bovine

测定

≥96% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

~66 kDa

纯化方式

cold ethanol fractionation

包装

poly bottle of

来源

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

缺失

≤6%

pH值(酸碱度)

6.5-7.5 (1% in 0.15 M sodium chloride)
7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

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应用

Bovine serum albumin has been used-
  • along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
  • as a component of modified Krebs-Ringer buffer (dmKRBT)
  • in gel shift analysis

包装

10, 50, 100, 500 g in poly bottle
1, 5 kg in poly drum

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

制备说明

通常称为 Cohn fraction V;本品采用改进的 Cohn 氏冷乙醇分级分离法制备。
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′s法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

请输入批号搜索分析证书(COA)。

原产地证书 (CofO)

请输入批号搜索原产地证书(COO)。

  1. Is Product A2153, Albumin from bovine serum (BSA), suitable for use in ELISA?

    We have not tested A2153 in quality control for use in ELISA applications. Because it contains globulins, we would generally not recommend A2153 for use in ELISA.

  2. Is Product A2153, Albumin from bovine serum (BSA), suitable for use in immunoblotting experiments as a blocking agent?

    We have not tested A2153 in quality control for use in immunoblotting applications as a blocking agent. Because it contains globulins, we would generally not recommend A2153 for use as a blocking agent.

  3. Product A2153, Albumin from bovine serum (BSA), powder looks tan in color.  Is this OK?

    The tan color is within specifications. The color can vary because of factors such as concentration of a solution at the time of lyophilization.

  4. Is Product A2153, Albumin from bovine serum (BSA), suitable for cell culture?

    We do not test A2153 for cell culture use, or for any specifications such as endotoxin content. Customers will have to exercise their own judgment as to whether they wish to use A2153 in cell culture, or can search the literature for publications that cite this product number.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Simple histochemical stain for acrosomes on sperm from several species.
Larson JL and Miller DJ
Molecular Reproduction and Development, 52(4), 445-449 (1999)
Bartolomeo Della Ventura et al.
PloS one, 12(2), e0171754-e0171754 (2017-02-10)
Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue
Mateusz Majda et al.
Developmental cell, 43(3), 290-304 (2017-11-08)
The epidermis of aerial plant organs is thought to be limiting for growth, because it acts as a continuous load-bearing layer, resisting tension. Leaf epidermis contains jigsaw puzzle piece-shaped pavement cells whose shape has been proposed to be a result
Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry.
Guthrie HD and Welch GR
Journal of Animal Science, 84(8), 2089-2100 (2006)
K Elaine Ritter et al.
Frontiers in neuroscience, 11, 690-690 (2018-01-10)
The autonomic and sensory nervous systems are required for proper function of all visceral organs, including the lower urinary tract (LUT). Despite the wide prevalence of bladder dysfunction, effective treatment options remain limited. Pelvic innervation regenerative strategies are promising, but

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