Merck
  • A3151
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A3151

Sigma-Aldrich

亲和素-过氧化物酶

lyophilized powder

Synonym(s):
亲和素-POD
MDL编号:
NACRES:
NA.46

生物来源

avidin from egg white
enzyme from horseradish

质量水平

偶联物

peroxidase conjugate

形式

lyophilized powder

组成

Protein, ≥70% E1%/280

标记范围

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

溶解性

H2O: soluble 1 mg/mL
PBS: soluble

储存温度

−20°C

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Avidin–Peroxidase buffered aqueous solution

Sigma-Aldrich

A7419

Avidin–Peroxidase

biological source

avidin from egg white, enzyme from horseradish

biological source

avidin from egg white, enzyme from horseradish

biological source

-

biological source

avidin from egg white, enzyme from bovine (calf) intestine

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

direct ELISA: 1:50,000

technique(s)

indirect ELISA: 1:100,000, western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

technique(s)

direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

亲和素是来自于蛋清的同源四聚体蛋白(68kDa)。这种蛋蛋白质与生物素高度结合。因此,亲和素­生物素结合已被用于免疫检测,以检测组织中的抗原定位。亲和素-生物素免疫检测的使用增强了该技术的灵敏度并改善了对低含量抗原的检测。

应用

为了检测小鼠血清中的Ga1缺陷型IgA1抗体,可在96孔板中使用血清进行ELISA检测,其中,血清与生物素化HAA(结合IgA1)一起孵育。然后将HRP偶联的亲和素与血清在37℃孵育40分钟。
亲和素-过氧化物酶已被用于检测抗5-溴脱氧尿苷(抗BrdU)结合和OVA-IgG1

外形

含有柠檬酸盐缓冲液的冻干粉末。

制备说明

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Product Information Sheet

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