Merck
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文件

A3156

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
9048-46-8
EC 号:
232-936-2
MDL编号:
MFCD00130384
NACRES:
NA.75

生物来源

bovine

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

检测方案

≥99% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

~66 kDa

纯化方式

cold ethanol and heatshock fractionation

来源

USA origin

technique(s)

cell culture | mammalian: suitable

杂质

≤10% water (Karl Fischer)

pH值（酸碱度）

4.8-7.5

溶解性

water: soluble (5g/50ml)

UniProt登记号

P02769

运输

ambient

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

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此商品
A2934A3608A2058
Bovine Serum Albumin lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture

Sigma-Aldrich

A3156

牛血清白蛋白 来源于牛血清

Bovine Serum Albumin lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A2934

牛血清白蛋白 来源于牛血清

Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A3608

牛血清白蛋白 来源于牛血清

Bovine Serum Albumin lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

Sigma-Aldrich

A2058

牛血清白蛋白 来源于牛血清

sterility

γ-irradiated

sterility

-

sterility

-

sterility

-

assay

≥99% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

assay

≥95% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

cold ethanol and heatshock fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

chromatography

一般描述

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Bovine serum albumin (BSA) is a 66 kDa protein consisting of three domains with two subdomains under each. It is a α-helical, globular and non-glycosylated protein. Albumin is the most abundant plasma protein in humans.

应用

Bovine serum albumin has been used:
  • to prevent sticking of the chromosome to the glass micropipette for elasticity measurements using aspiration
  • to block lung homogenates in the plate for mucin protein enzyme-linked immunosorbent assay
  • in alginate solution as a protein model for drug release kinetics investigation

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. Albumin turnover is seen in infants with iron deficiency anemia. BSA acts as a vital constituent in the cell culture media and favors human embryonic stem cells (hESC) differentiation.
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克和乙醇分级分离相结合的方法进行纯化

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Elasticity and Structure of Eukaryote Chromosomes Studied by Micromanipulation and Micropipette Aspiration
Houchmandzadeh B, et al.
The Journal of cell biology, 139(1), 1-12 (1997)
Acrolein-Activated Matrix Metalloproteinase 9 Contributes to Persistent Mucin Production
Deshmukh HS, et al.
American Journal of Respiratory Cell and Molecular Biology (2012)
Combination of polymer technology and carbon nanotube array for the development of an effective drug delivery system at cellular level
Riggio C, et al.
Nanoscale Research Letters, 4(7), 668-668 (2009)
Albumin and mammalian cell culture: implications for biotechnology applications
Francis GL
Cytotechnology, 62(1), 1-16 (2010)
Bovine serum albumin interactions with metal complexes
Topalua T, et al.
Clujul Medical (1957), 87(4), 215-215 (2014)
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This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

白蛋白在细胞培养中的应用

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能，并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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