Merck

A3294

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, protease free, pH 7, ≥98%

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
EC 号:
MDL编号:
NACRES:
NA.27

生物来源

bovine

检测方案

≥98%

形式

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

poly bottle of

来源

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

杂质

protease, none detected

缺失

≤5%

pH值(酸碱度)

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

BT Virus, none detected
VSV Virus, none detected

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

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此商品
A9056A7638A2153
assay

≥98%

assay

≥96% (agarose gel electrophoresis)

assay

≥99% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

cold ethanol and heatshock fractionation

purified by

cold ethanol fractionation

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

一般描述

白蛋白大量存在于人血浆中。白蛋白浓度升高会造成脱水。患有缺铁性贫血的儿童常见有白蛋白周转异常。可作为肝病的预测性指标。

应用

牛血清白蛋白已用于:
  • 封闭抗体的非特异性结合
  • 作为精子活力激发培养基组分
  • 通过二辛可宁酸(BCA) 方法测定细菌素的蛋白浓度

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特点和优势

  • 热休克分级
  • 无蛋白酶

制备说明

热激分级分离法制取
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Leuconostoc carnosum 4010 has the potential for use as a protective culture for vacuum-packed meats: culture isolation, bacteriocin identification, and meat application experiments
Budde B B, et al.
International Journal of Food Microbiology, 83(2), 171-184 (2003)
Histomorphometric alteration and cell-type specific modulation of arylhydrocarbon receptor and estrogen receptor expression by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and 17beta-estradiol in mouse experimental model of endometriosis
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Cryopreservation of Atlantic cod Gadus morhua L. spermatozoa: effects of extender composition and freezing rate on sperm motility, velocity and morphology
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Cutaneous squamous cell carcinomas (cSCC) are the primary cause of premature deaths in patients suffering from the rare skin-fragility disorder recessive dystrophic epidermolysis bullosa (RDEB), which is in marked contrast to the rarely metastasizing nature of these carcinomas in the
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