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Merck

A3525

Sigma-Aldrich

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

别名:

Goat Anti-Human IgE (ε-chain specific)−AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

human

技術

direct ELISA: 1:7,000-1:21,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

Affinity isolated antibody is obtained from anti-human IgE antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the ε-chain of human IgE. Anti-Human IgE is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde. IgE is present in low levels compared to other immunoglobulin isotypes. It exists in two forms, one being a secreted by plasma cell and the other form is associated with the B cell receptor.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma . Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is specific for human IgE when tested against human IgA, IgG, IgM, Bence Jones κ and λ myeloma proteins.

免疫原

Purified human IgE

應用

Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat has been used in dot blot.
Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is suitable for use in immunoblotting and ELISA (at 1:500 dilutions using 100μ/well).
IgE secreted from transfected murin myeloma cells was detected by ELISA using the alkaline phosphatase-conjugated goat anti-human IgE (epsilon specific) antibody.

生化/生理作用

IgE bound to B cell receptor mediates antigen uptake and regulates differentiation and proliferation of B cells.

外觀

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析证书(COA)

Lot/Batch Number

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Juliette Delaunay et al.
Contact dermatitis, 78(4), 291-292 (2018-03-13)
Dalgys Martínez et al.
Immunology letters, 196, 103-112 (2018-02-07)
Sensitization to allergens of the house dust mites Dermatophagoides pteronyssinnus and Blomia tropicalis is an important risk factor for asthma and allergic diseases. Allergen-specific immunotherapy is currently based on natural allergen extracts, however, in the last years recombinant allergens with
Jack H Jhamandas et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(24), 5579-5584 (2004-06-18)
Salvage of cholinergic neurons in the brain through a blockade of the neurotoxic effects of amyloidbeta protein (Abeta) is one of the major, but still elusive, therapeutic goals of current research in Alzheimer's disease (AD). To date, no receptor has
L K Ellertsen et al.
International archives of allergy and immunology, 138(3), 217-224 (2005-10-08)
A negative association has been observed between infections and allergy in several studies. The aim of the present study was to examine whether tuberculosis and leprosy patients have more or fewer allergies than healthy individuals. Sera from tuberculosis patients, leprosy
Yanfang Rui et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(41), 10480-10487 (2006-10-13)
Defects in axonal transport are often associated with a wide variety of neurological diseases including Alzheimer's disease (AD). Beta-amyloid (Abeta) is a major component of neuritic plaques associated with pathological conditions of AD brains. Here, we report that a brief

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