Merck
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文件

A4327

Sigma-Aldrich

白蛋白 来源于人类血清

lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

所有图片(2)
别名:
HSA
CAS号:
70024-90-7
EC 号:
274-272-6
MDL编号:
MFCD00081418
NACRES:
NA.25

生物来源

human

质量水平

200

检测方案

≥96% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

monomer calculated mol wt 66478 Da

technique(s)

tissue culture: suitable

杂质

HIV I and HIVII, HCV and HBsAg, tested negative

溶解性

H2O: soluble 50 mg/mL

UniProt登记号

P02768

异质活性

Alkaline phosphatase ≤0.001 units/mg protein
Peroxidase ≤0.001 units/mg protein
Protease ≤0.0001 units/mg protein

储存温度

2-8°C

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

InChI key

QYSGYZVSCZSLHT-UHFFFAOYSA-N

Gene Information

human ... ALB(213)

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此商品
A1887A9511A3782
Albumin from human serum lyophilized powder, essentially protease free, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A4327

白蛋白 来源于人类血清

Albumin from human serum lyophilized powder, essentially fatty acid free

Sigma-Aldrich

A1887

白蛋白 来源于人类血清

Albumin from human serum lyophilized powder, ≥97% (agarose gel electrophoresis)

Sigma-Aldrich

A9511

白蛋白 来源于人类血清

Albumin from human serum lyophilized powder, Fatty acid free, Globulin free, ≥99% (agarose gel electrophoresis)

Sigma-Aldrich

A3782

白蛋白 来源于人类血清

assay

≥96% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

assay

≥97% (agarose gel electrophoresis)

assay

≥99% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

technique(s)

tissue culture: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

impurities

HIV I and HIVII, HCV and HBsAg, tested negative, ≤0.007% Fatty Acids

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

impurities

HIV I and HIVII, HCV and HBsAg, tested negative, ≤0.1% Fatty acids

一般描述

Albumin is the most copious protein in blood plasma. Produced in the liver, serum albumin serves as a carrier protein for biomolecules like as fatty acids, amino acids, and steroids.

This human serum albumin (HSA) product has been specially tested and evaluated to be low in the following properties:
  • Essentially protease-free / low protease content
  • Low alkaline phosphatase content
  • Low peroxidase content

Serum albumin is used in various applications related to molecular biology, notably as an inert protein stabilizer for stock solutions of enzymes and proteins of interest. In such applications, it is thus important for the HSA to be protease free, to maintain the integrity of the protein or enzyme of interest.

Protease-free HSA is especially useful for applications like:
  • Enzyme assays
  • Protein-based assays
  • Protease-sensitive techniques such as:
(a) Enzyme immunoassay (EIA)
(b) Nucleic acid hybridization
(c) Radioimmunoassay (RIA)

应用

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia. It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.
It was used to test the effect of non-enzymatic glycation on the unfolding of human serum albumin. It was also used to test the two distinct pathways used by intestinal epithelial cells for HLA class II antigen processing.

生化/生理作用

血清白蛋白可作为类固醇、脂肪酸和甲状腺激素的载体蛋白，对调节血液的胶体渗透压至关重要。研究还发现白蛋白可结合外源物质，特别是药物（例如布洛芬、华法林），并强烈影响其药代动力学。导致白蛋白氧化还原状态变化的氧化应激对其生理功能具有广泛的影响。

特点和优势

  • Low protease activity / essentially protease-free (≤ 0.0001 units/mg protein)
  • Low alkaline phosphatase activity (≤ 0.001 units/mg protein)
  • Low peroxidase activity (≤ 0.001 units/mg protein)

制备说明

Prepared from A1653

其他说明

更多信息参见人血清白蛋白。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves

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H0900000

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Current protocols in stem cell biology, Chapter 1, Unit 1A-Unit 1A (2008-09-05)
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The Journal of clinical investigation, 100(1), 204-215 (1997-07-01)
Intestinal epithelial cells express a low level of HLA class II molecules constitutively, with elevated levels seen in the setting of mucosal inflammation including inflammatory bowel disease. The ability of intestinal epithelial cells to act as antigen presenting cells for
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