A4470

Sigma-Aldrich

Monoclonal Anti-Adenovirus 2 E1A (Adenovirus 2 early region 1) antibody produced in mouse

1.0 mg/mL, clone M73, purified immunoglobulin, buffered aqueous solution

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M73, monoclonal

form

buffered aqueous solution

mol wt

antigen 30-50 kDa (3 bands)

species reactivity

viral

concentration

1.0 mg/mL

application(s)

immunoprecipitation (IP): 1-2 μg/mL using whole extract of HEK 293 cells
indirect immunofluorescence: 2-5 μg/mL

isotype

IgG2a

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

General description

Adenovirus E1A gene modulates gene expression by associating with transcription factors in cells. Studies have reported that E1A regulates the stimulation of ICAM-1 through inflammatory signals in pulmonary epithelial cells. E1A has also been implicated in chronic obstructive pulmonary disease (COPD). Furthermore, E1A is known to mediate tumor suppression by rendering epithelial cells sensitive to anoikis. Mouse Monoclonal Anti-Adenovirus 2 E1A antibody binds to adenovirus 2 and 5 E1A (3 bands at 30-50kDa by SDS-PAGE), as well as E1A associated proteins.

Immunogen

full length recombinant adenovirus 2 E1A protein.

Application

Monoclonal Anti-Adenovirus 2 E1A can be used for indirect immunofluorescence (2-5μg/mL) and immunoprecipitation (1-2μg/mL, using whole extract of HEK 293 cells).

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
E Harlow et al.
Molecular and cellular biology, 6(5), 1579-1589 (1986-05-01)
Extracts from adenovirus-transformed human 293 cells were immunoprecipitated with monoclonal antibodies specific for the early-region 1A (E1A) proteins. In addition to the E1A polypeptides, these antibodies precipitated a series of proteins with relative molecular weights of 28,000, 40,000, 50,000, 60,000...
N Keicho et al.
American journal of respiratory cell and molecular biology, 16(1), 23-30 (1997-01-01)
Previous studies from our laboratory demonstrated that adenovirus E1A DNA and proteins are detected in lungs of patients with chronic obstructive pulmonary disease (COPD). Since adenovirus E1A gene products are known to regulate the expression of many genes by interacting...
E Harlow et al.
Journal of virology, 55(3), 533-546 (1985-09-01)
Hybridomas secreting monoclonal antibodies specific for the adenovirus early region 1A (E1A) proteins were prepared from BALB/c mice immunized with a bacterial trpE-E1A fusion protein. This protein is encoded by a hybrid gene that joins a portion of the Escherichia...
Li-Li Guo et al.
Experimental and therapeutic medicine, 15(6), 5394-5402 (2018-05-31)
Viral vectors represent a potential strategy for the treatment of human malignant tumors. Currently, recombinant adenovirus vectors are commonly used as gene therapy vehicles, as it possesses a proven safety profile in normal human cells. The recombinant adenovirus system has...
S M Frisch
Mutation research, 350(1), 261-266 (1996-02-19)
Tumor suppressor genes such as Rb and p53 usually kill tumor cells when overexpressed ectopically. This is a consequence of their normal cell cycle regulatory functions. By contrast, the E1a gene of adenovirus, a common cold virus, converts tumor cells...

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