Merck

A4541

Sigma-Aldrich

抗-生物素−过氧化物酶 山羊抗

affinity isolated antibody, buffered aqueous solution

登录to View Organizational & Contract Pricing

别名:
抗-生物素
NACRES:
NA.46

生物来源

goat

质量水平

偶联物

peroxidase conjugate

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

正在寻找类似产品? Visit 产品对比指南

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
A7064B3640A1559
Sigma-Aldrich

Sigma-Aldrich

A7064

抗-生物素

Sigma-Aldrich

Sigma-Aldrich

B3640

抗-生物素 山羊抗

Sigma-Aldrich

Sigma-Aldrich

A1559

单克隆抗-生物素

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

BN-34, monoclonal

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

PBS suspension

technique(s)

direct ELISA: 1:2,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

immunoelectrophoresis: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

一般描述

生物素是活生物体和培养物中细胞所需的必需维生素。生物素与蛋白亲和素或细菌来源的链霉亲和素的高结合亲和力已被用于设计用于检测抗原与抗体的相互作用的免疫测定法。山羊抗生物素过氧化物酶抗体可用于许多可能将生物素引入靶标的应用。
通过 Ouchterlony 双扩散和免疫电泳与生物素载体蛋白复合物测定特异性和反应性。未观察到与载体蛋白的反应性。

免疫原

生物素-KLH 结合物

应用

使用 HRP 标记的山羊抗生物素抗体,通过蛋白质印迹分析 U937 和 BHK 细胞裂解物中的生物素标记蛋白。
在一些应用中,生物素化探针与亲和素的定位会产生高背景水平。抗生物素试剂可替代亲和素,以减少非特异性结合。
山羊抗生物素过氧化物酶抗体可用于直接 ELISA 分析(稀释度为 1:2,000)。

外形

0.01 M磷酸盐缓冲液,pH 7.4,含有1%牛血清白蛋白和防腐剂。

制备说明

通过 Wilson,M.B. 和 Nakane,P.K. 在《免疫荧光及相关染色技术》(Immunofluorescence and Related Staining Techniques) 中描述的过碘酸盐法制备(Elsevier/North Holland Biomedical Press 出版社,Amsterdam,p215 (1978))。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形图

Exclamation markEnvironment

警示用语:

Warning

危险分类

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

其他客户在看

Slide 1 of 8

1 of 8

Sigma-Aldrich

Sigma-Aldrich

A3151

亲和素-过氧化物酶

Anti-Fluorescein-POD, Fab fragments from sheep

Roche

11426346910

抗荧光素-POD Fab片段

Streptavidin-HRP Conjugate Cytiva RPN1231-2ML

GERPN1231-2ML

Streptavidin-HRP Conjugate

DAB Substrate dark brown, /black precipitate; Visually evaluated

Roche

11718096001

DAB 底物

Biotin ≥99% (HPLC), lyophilized powder

Sigma-Aldrich

B4501

生物素

Katarzyna Kwiatkowska et al.
Journal of cell science, 116(Pt 3), 537-550 (2003-01-01)
Activation of Fcgamma receptor II (FcgammaRII) induces rearrangement of the actin-based cytoskeleton that serves as a driving force for FcgammaRII-mediated phagocytosis and FcgammaRII capping. To get insight into the signaling events that lead to the actin reorganization we investigated the
Soledad Astrada et al.
Journal of peptide science : an official publication of the European Peptide Society, 22(11-12), 711-722 (2016-12-10)
Because of resistance development by cancer cells against current anticancer drugs, there is a considerable interest in developing novel antitumor agents. We have previously demonstrated that CIGB-552, a novel cell-penetrating synthetic peptide, was effective in reducing tumor size and increasing
Samuel H Henager et al.
Nature methods, 13(11), 925-927 (2016-11-01)
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-terminal cysteine (N-Cys)-containing peptides, but the requirement of a Cys residue at the ligation junction can limit the utility of
Nataliya Gorinski et al.
The Journal of biological chemistry, 295(18), 5970-5983 (2020-03-19)
Barttin is the accessory subunit of the human ClC-K chloride channels, which are expressed in both the kidney and inner ear. Barttin promotes trafficking of the complex it forms with ClC-K to the plasma membrane and is involved in activating
Ee Lin Wong et al.
Nature communications, 10(1), 66-66 (2019-01-10)
Protein-templated fragment ligations have been established as a powerful method for the assembly and detection of optimized protein ligands. Initially developed for reversible ligations, the method has been expanded to irreversible reactions enabling the formation of super-additive fragment combinations. Here

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门