A5503

Sigma-Aldrich

白蛋白 来源于鸡蛋白

lyophilized powder, ≥98% (agarose gel electrophoresis)

别名:
清蛋白 来源于鸡蛋白, 卵清蛋白
CAS号:
EC 号:
MDL编号:
eCl@ss:
42010201
NACRES:
NA.25

质量水平

300

生物来源

chicken egg white

类型

Grade V

测定

≥98% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

44287 Da by calculation

纯化方式

crystallization

应用

bioconjugation: suitable

溶解性

H2O: soluble 40 mg/mL

UniProt登记号

储存温度

2-8°C

Gene Information

chicken ... OVAL(396058)

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一般描述

鸡蛋清清蛋白(或卵清蛋白)是蛋清的主要蛋白质成分。鸡蛋清白蛋白是磷酸化糖蛋白。根据氨基酸序列,卵清蛋白的肽部分由 385 个残基组成,分子量为 42.7kDa。

应用

卵清蛋白可用作蛋白质电泳的标记物(∼45 kDa)。卵清蛋白已用于构建哮喘的动物模型。卵清蛋白已用作EliCell测定中细胞培养基,用于检测由嗜酸性粒细胞释放的细胞因子。
研究DNA免疫分布影响的研究在抗体测定过程使用了卵清蛋白。
卵清蛋白已被用作特应性皮炎模型研究的诱导剂。
磷酸化糖蛋白,可用作蛋白质电泳的标记物(~45kDa)。已被用于生产哮喘的动物模型。

包装

1, 5, 10, 25, 50 g in poly bottle

生化/生理作用

差示扫描量热法表明鸡蛋清蛋白会在84℃发生变性。卵清蛋白可用作载体蛋白以与合成肽偶联以用作免疫原。

外形

基本不含盐

象形图

Health hazard

警示用语:

Danger

危险声明

hazcat

Resp. Sens. 1A

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

  1. Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?

    A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.

  2. What is the solubility of Product No. A5503, Albumin from chicken egg white, in aqueous buffers (e.g. Tris, PBS)?

    We have only tested solubility in water. Salts in buffers may make solubility more difficult.

  3. How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?  

    A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE.

  4. Product No. A5503, Albumin from chicken egg white, is to be stored at 2 - 8°C. Does it ship on ice?

    A5503 does not require shipping on ice.

  5. Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?

    We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J S Boyle et al.
International immunology, 9(12), 1897-1906 (1998-02-18)
We examined the role of the cellular localization of antigen on the immune response after DNA immunization of mice with three forms of ovalbumin (OVA). DNA encoding OVA which was secreted (sOVA) generated 10- to 100-fold higher IgG responses with...
Ken Morishima et al.
Communications biology, 3(1), 294-294 (2020-06-10)
Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different...
Christianne Bandeira-Melo et al.
Journal of immunological methods, 276(1-2), 227-237 (2003-05-10)
Eosinophils contain several preformed cytokines within their specific granules. Therefore, without requiring them in de novo synthesis of cytokines, eosinophils can release quantities of granule-derived cytokines by highly regulated mechanisms. However, eosinophil "degranulation" is poorly understood, in part, because available...
A D Nisbet et al.
European journal of biochemistry, 115(2), 335-345 (1981-04-01)
The complete amino acid sequence of hen ovalbumin, comprising 385 residues, has been determined. The sequence was deduced from the 17 cyanogen bromide fragments and from peptides derived by digestion with a number of proteolytic enzymes. The molecular weight of...
Ana Raquel Maceiras et al.
Nature communications, 8, 15067-15067 (2017-04-22)
Immunization leads to the formation of germinal centres (GCs) that contain both T follicular helper (Tfh) and T follicular regulatory (Tfr) cells. Whether T-cell receptor (TCR) specificity defines the differential functions of Tfh and Tfr cells is unclear. Here we...
技术文章
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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