Merck
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A6964

Sigma-Aldrich

Accutase® 溶液

sterile-filtered, suitable for cell culture

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Synonym(s):
Cell detachment solution
MDL编号:
MFCD01632905
NACRES:
NA.75

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Accutase cell detachment solution A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

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SCR005

蛋白酶细胞分离液

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ESGRO Complete 细胞消化液

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胰蛋白酶-EDTA 溶液

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Enzyme Free Cell Dissociation Solution PBS Based (1X), liquid, 500ml The Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.

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The Enzyme-Free Cell Dissociation Solution is a PBS based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 500ml format.

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T3924

胰蛋白酶-EDTA 溶液

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二甲基亚砜

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sterility

sterile-filtered

质量水平

200

形式

liquid

浓度

400-600 units/mL

technique(s)

cell culture | mammalian: suitable

pH值（酸碱度）

6.8-7.8

运输

dry ice

储存温度

−20°C

Related Categories

一般描述

Accutase®溶液（在不含Ca ++或Mg ++的杜氏磷酸缓冲盐溶液(DPBS)）包含用于细胞解离的蛋白水解酶和胶原蛋白水解酶。可用于从标准组织培养塑料器具和粘附涂层塑料器具中常规解离细胞。

应用

Accutase®溶液已用于将聚集簇中生长的非贴壁细胞（如脑肿瘤细胞、诱导性多能干细胞(IPSC)和胶质母细胞瘤细胞）解离或酶解成单个细胞。
证明可有效对原代成纤维细胞、内皮细胞、神经元、肿瘤细胞系和昆虫细胞进行解离。 在用于分析细胞表面标志物、病毒生长检测、流式细胞术以及生物反应器放大的细胞解离中表现尤为突出。不含有哺乳动物或细菌来源产品。

包装

100, 500 mL

特点和优势

由蛋白水解酶和胶原溶解酶的专有混合所组成的即用型液体用于将细胞从标准和带有粘附涂层的塑料器皿上进行解离。

公式变量

制备采用含0.5 mM EDTA•4Na以及3 mg/L酚红的Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl以及1.15 g/L Na2HPO4)。 可参考网站上的产品数据清单获取产品用途信息。

制备说明

该产品中含有酚红。该产品通过干冰进行运输所有在包装中会有显著的CO2累积。这些CO2可能会进入到溶液并略微降低pH，从而从淡粉色（约pH 7.8）转化为黄橘色（约pH 6.8）。该溶液如果呈现黄橘色（酸性）则仍然可以正常使用，或加入氢氧化钠对pH进行调节。

法律信息

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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