Merck

A6964

Sigma-Aldrich

Accutase® 溶液

sterile-filtered, suitable for cell culture

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别名:
Cell detachment solution
MDL编号:
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

形式

liquid

浓度

400-600 units/mL

技术

cell culture | mammalian: suitable
single cell analysis: suitable

pH值(酸碱度)

6.8-7.8

运输

dry ice

储存温度

−20°C

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此商品
SCR005SCR104A7089
Accutase® 溶液 sterile-filtered, suitable for cell culture

A6964

Accutase® 溶液

蛋白酶细胞分离液 A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

SCR005

蛋白酶细胞分离液

Accutase®XL Lyophilized Accutase AccutaseXL is a lyophilized form of Accutase with extended stability used for the routine detachment of cells from standard tissue culture plasticware

SCR104

Accutase®XL Lyophilized Accutase

ACCUMAX™ 溶液 sterile-filtered, suitable for cell culture

A7089

ACCUMAX 溶液

form

liquid

form

liquid

form

liquid

form

solution

concentration

400-600 units/mL

concentration

-

concentration

-

concentration

1 ×

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

sterility

sterile-filtered

sterility

-

sterility

-

sterility

sterile-filtered

pH

6.8-7.8

pH

-

pH

-

pH

6.8-7.8

一般描述

Accutase®溶液(在不含Ca ++或Mg ++的杜氏磷酸缓冲盐溶液(DPBS))包含用于细胞解离的蛋白水解酶和胶原蛋白水解酶。可用于从标准组织培养塑料器具和粘附涂层塑料器具中常规解离细胞。Accutase 是优于胰蛋白酶的细胞脱壁试剂,因为它有助于细胞从培养容器分离,损伤较少。因此,Accutase 常用于处理敏感的细胞或用于分析表面标志物。

应用

Accutase®溶液已用于将聚集簇中生长的非贴壁细胞(如脑肿瘤细胞、诱导性多能干细胞(IPSC)和胶质母细胞瘤细胞)解离或酶解成单个细胞。

适合制备测序用单细胞悬液。
证明可有效对原代成纤维细胞、内皮细胞、神经元、肿瘤细胞系和昆虫细胞进行解离。 在用于分析细胞表面标志物、病毒生长检测、流式细胞术以及生物反应器放大的细胞解离中表现尤为突出。不含有哺乳动物或细菌来源产品。

特点和优势

由蛋白水解酶和胶原溶解酶的专有混合所组成的即用型液体用于将细胞从标准和带有粘附涂层的塑料器皿上进行解离。

公式变量

制备采用含0.5 mM EDTA•4Na以及3 mg/L酚红的Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl以及1.15 g/L Na2HPO4)。 可参考网站上的产品数据清单获取产品用途信息。

制备说明

该产品中含有酚红。该产品通过干冰进行运输所有在包装中会有显著的CO2累积。这些CO2可能会进入到溶液并略微降低pH,从而从淡粉色(约pH 7.8)转化为黄橘色(约pH 6.8)。该溶液如果呈现黄橘色(酸性)则仍然可以正常使用,或加入氢氧化钠对pH进行调节。

法律信息

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

相关产品

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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