sterility
sterile-filtered
Quality Level
form
solution
concentration
1 ×
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
pH
6.8-7.8
shipped in
dry ice
storage temp.
−20°C
Application
Accumax已经证明能够在杂交瘤、CHO、BHK、293、COS和Sf9细胞的悬浮培养物中对成簇细胞进行有效解离。 该溶液在解离细胞计数、病毒转染测定、细胞分选、流式细胞术和生物反应器放大方面具有极佳的表现。 它还被用于从聚集的细胞培养物中获得单细胞悬浮液，以实现精确的细胞计数、细胞与原发组织的分离、以及细胞与标准组织培养塑料器皿和粘附涂层塑料器皿的常规分离。
TM = [“Accumax”]溶液已用于
- 从上皮细胞的细胞外基质(ECM)解离单壁碳纳米管
- 在传代前解离视网膜色素层细胞
- 从胰腺上解离胰岛细胞
Biochem/physiol Actions
Accumax是一种细胞分离溶液，由蛋白水解酶和胶原溶解酶组成。 每瓶含有100 mL溶于杜氏′PBS的1x Accumax酶。Accumax中没有发现哺乳动物或细菌衍生产物。
Features and Benefits
用于体外 细胞应用的即用型无菌液体
Preparation Note
在杜氏′PBS（0.2g/L KCl、0.2g/L KH2PO4、8g/L NaCl和1.15g/L Na2HPO4）中进行制备。 有关产品使用信息，请参阅网站上的产品数据表。
本产品以无菌即用型冷冻液的形式提供。 Accumax应在使用前在37°C解冻，并以等体积加入至集聚细胞样品中。 在37°C下孵育5-10分钟，然后进行正常计数细胞。
Legal Information
Accumax is a trademark of Innovative Cell Technologies, Inc.
Disclaimer
Accumax在-20°C下储存可保持稳定。解冻后，产品可在4°C下储存长达2个月。
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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相关内容
Datasheet
Benjamin T Dodsworth et al.
Scientific reports, 10(1), 10542-10542 (2020-07-01)
Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis.
Chronic beta-cell depolarization impairs beta-cell identity by disrupting a network of ca2+-regulated genes
Stancill JS, et al.
Diabetes, 66(8), 2175-2187 (2017)
Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration
Geng Z, et al.
PLoS ONE, 12(3), e0173575-e0173575 (2017)