Merck
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A7089

Sigma-Aldrich

ACCUMAX™ 溶液

sterile-filtered, suitable for cell culture

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Synonym(s):
Cell detachment solution
MDL编号:
MFCD01632904
NACRES:
NA.75

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sterility

sterile-filtered

质量水平

200

形式

solution

浓度

1 ×

technique(s)

cell culture | mammalian: suitable

pH值（酸碱度）

6.8-7.8

运输

dry ice

储存温度

−20°C

Related Categories

应用

TM = [“Accumax”]溶液已用于
  • 从上皮细胞的细胞外基质(ECM)解离单壁碳纳米管
  • 在传代前解离视网膜色素层细胞
  • 从胰腺上解离胰岛细胞

Accumax已经证明能够在杂交瘤、CHO、BHK、293、COS和Sf9细胞的悬浮培养物中对成簇细胞进行有效解离。 该溶液在解离细胞计数、病毒转染测定、细胞分选、流式细胞术和生物反应器放大方面具有极佳的表现。 它还被用于从聚集的细胞培养物中获得单细胞悬浮液，以实现精确的细胞计数、细胞与原发组织的分离、以及细胞与标准组织培养塑料器皿和粘附涂层塑料器皿的常规分离。

包装

1 ea
100, 500 mL

生化/生理作用

Accumax是一种细胞分离溶液，由蛋白水解酶和胶原溶解酶组成。 每瓶含有100 mL溶于杜氏′PBS的1x Accumax酶。Accumax中没有发现哺乳动物或细菌衍生产物。

特点和优势

用于体外 细胞应用的即用型无菌液体

注意

Accumax在-20°C下储存可保持稳定。解冻后，产品可在4°C下储存长达2个月。

公式变量

在杜氏′PBS（0.2g/L KCl、0.2g/L KH2PO4、8g/L NaCl和1.15g/L Na2HPO4）中进行制备。 有关产品使用信息，请参阅网站上的产品数据表。

制备说明

本产品以无菌即用型冷冻液的形式提供。 Accumax应在使用前在37°C解冻，并以等体积加入至集聚细胞样品中。 在37°C下孵育5-10分钟，然后进行正常计数细胞。

法律信息

Accumax is a trademark of Innovative Cell Technologies, Inc.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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