Merck
  • A7294
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A7294

Sigma-Aldrich

抗生物素蛋白碱性磷酸酶

buffered aqueous solution

Synonym(s):
Avidin–AP
MDL编号:
NACRES:
NA.46

生物来源

avidin from egg white
enzyme from bovine (calf) intestine

质量水平

偶联物

alkaline phosphatase conjugate

形式

buffered aqueous solution

technique(s)

direct ELISA: 1:70,000
western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

运输

wet ice

储存温度

2-8°C

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This Item
A2050E2636A3151
biological source

avidin from egg white, enzyme from bovine (calf) intestine

biological source

-

biological source

-

biological source

avidin from egg white, enzyme from horseradish

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

direct ELISA: 1:50,000

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

一般描述

亲和素-生物素系统用于免疫分析,检测组织中抗原位置。使用亲和素-生物素免疫分析增强了技术的灵敏度,能够检测出少量的抗原。
亲和素是从蛋清中获得的同四聚体蛋白(66 kDa),可与生物素牢固结合。它有四个相同的亚基,各为16,400道尔顿。它是一种有吸引力的衔接蛋白,可抵抗变性。这种糖蛋白存在于鸟类、爬行动物和两栖动物的蛋清中。该产品是通过0.2%戊二醛法与碱性磷酸酶偶联的亲和纯化的蛋清亲和素(活性为10-15个单位/mg蛋白)。

应用

亲和素碱性磷酸酶可用于ELISA该产品也可按1:150,000-1:300,000稀释度用于蛋白质印迹。
亲和素碱性磷酸酶可用于酶联免疫吸附测定(ELISA)。
使用生物素化抗IL-2抗体和碱性磷酸酶亲和素进行细胞因子ELISA分析。

生化/生理作用

亲和素-生物素系统用于免疫分析,检测组织中抗原位置。使用亲和素-生物素免疫分析增强了技术的灵敏度,能够检测出少量的抗原。亲和素是一种脂肪酸生物合成调节剂。它通过削弱细胞增殖而不影响分化过程,从而参与细胞终末分化。

外形

pH 8.0、含1%牛血清白蛋白、1 mM MgCl 2 和15 mM叠氮化钠的0.05 M Tris缓冲液溶液

制备说明

通过0.2%戊二醛法与碱性磷酸酶偶联的亲和纯化蛋白。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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