Merck

A7419

Sigma-Aldrich

Avidin–Peroxidase

buffered aqueous solution

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别名:
Avidin–HRP
MDL编号:
NACRES:
NA.46

生物来源

avidin from egg white
enzyme from horseradish

质量水平

偶联物

peroxidase conjugate

形式

buffered aqueous solution

技术

indirect ELISA: 1:100,000
western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)

运输

dry ice

储存温度

−20°C

一般描述

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

应用

Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.
Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

制备说明

Affinity purified protein.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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