Merck
All Photos(1)

A8531

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

pH <5.0, powder

Synonym(s):
BSA, 牛白蛋白, 牛血清白蛋白
CAS Number:
MDL编号:
eCl@ss:
42010201
NACRES:
NA.56

生物来源

bovine

质量水平

200

形式

powder

分子量

~66 kDa
~66,000

包装

vial of 50 mg

technique(s)

ELISA: suitable
gel permeation chromatography (GPC): suitable
immunohistochemistry: suitable

杂质

~0.3% dithiothreitol

pH值(酸碱度)

<5.0

适用性

suitable for (Suitable for use as a gel filtration marker)

UniProt登记号

储存温度

−20°C

Gene Information

bovine ... ALB(280717)

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一般描述

Albumin is the most abundant plasma protein in humans. It has a molecular mass of 66.5 kDa.

应用

Albumin from bovine serum has been used:
  • for blocking of nonspecific immunoglobulin during immunohistochemical reactions.
  • to coat microplates for blocking non-specific binding during I-ELISA (Indirect Enzyme-linked Immunosorbent Assay)
  • as molecular mass standard in analytical gel filtration chromatography for Superdex® 75 10/300 GL gel filtration column.
  • for measuring fluorescence for PR1 (phytofluor red 1) by using Fluorescence correlation spectroscopy (FCS)
  • as a reference mixture along with horse Mb in Size Exclusion Chromatography
It can be used as a gel filtration molecular weight marker. It can be used in medical studies to quantify binding capacity and adsorption rate of protein enrichment and separation medium critical in proteomic research.
Gel filtration molecular weight marker

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also triggers the insulin dependent diabetes mellitus.
High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Albumin in serum level is associated with advanced liver disease.

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

法律信息

Superdex is a registered trademark of Cytiva

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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