Merck

A8806

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)

登录to View Organizational & Contract Pricing

Select a Size

别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
EC 号:
MDL编号:
NACRES:
NA.27

生物来源

bovine

质量水平

产品线

BioReagent

检测方案

≥96% (agarose gel electrophoresis)

形式

lyophilized powder

分子量

~66 kDa

纯化方式

cold ethanol fractionation

包装

poly bottle of

来源

USA origin

free fatty acid content

≤0.02%

technique(s)

cell culture | mammalian: suitable

杂质

≤0.02% fatty acid (GC)
≤0.1 ng/mg endotoxin

缺失

≤5%

pH值(酸碱度)

4.8-7.5

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

AVA 9CFR 113.53, none detected

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

正在寻找类似产品? Visit 产品对比指南

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
A2058A4919A1933
assay

≥96% (agarose gel electrophoresis)

assay

≥95% (agarose gel electrophoresis)

assay

≥98%

assay

≥98%

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

cold ethanol fractionation

purified by

chromatography

purified by

heat shock fractionation

purified by

chromatography

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

一般描述

牛血清白蛋白(BSA) 是一种水溶性血清蛋白,长度为 583 个氨基酸,计算分子量为 66,430 道尔顿。六个α-螺旋形成其三个同源结构域。牛血清白蛋白随pH变化经历可逆的构象异构化。天然BSA 结构在加热时具有反应性和灵活性。

BSA 是脂肪酸、氨基酸和类固醇等生物分子的载体蛋白。由于其载体特性,BSA也被广泛用于细胞培养。在用于细胞培养时BSA 还具有抗氧化功能。

应用

牛血清白蛋白已被用于为组织消化而制备的溶液中。它已被用作细胞铺展测定中的阻断剂。

由于 BSA 是众所周知的脂肪酸(FA)载体,因此,对于对特定脂肪酸敏感的细胞培养系而言,控制细胞系培养用特定脂肪酸很重要。不含脂肪酸的 BSA 是此类细胞培养实验中的有用试剂,可以让研究人员使用此类 BSA 作为其细胞培养系统特有的特定脂肪酸的载体。这减轻了非无 FA BSA 中可能存在的 FA′ 对细胞的潜在风险。

该 BSA 产品针对以下特性进行了专门测试:
  • 不含脂肪酸/FA 含量非常低
  • 内毒素水平低
  • 细胞培养的适用性

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特点和优势

  • 经测试可用于细胞培养
  • 无脂肪酸
  • 低内毒素
  • 冷乙醇分级

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为第五组分(Cohn fraction V);本品采用改进的Cohn氏冷乙醇分级分离法制备。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

Hajime Abe et al.
Nature communications, 10(1), 2824-2824 (2019-06-30)
The fibrogenic response in tissue-resident fibroblasts is determined by the balance between activation and repression signals from the tissue microenvironment. While the molecular pathways by which transforming growth factor-1 (TGF-β1) activates pro-fibrogenic mechanisms have been extensively studied and are recognized
Extracellular stimulation of mammalian neurons through repetitive activation of Na+ channels by weak capacitive currents on a silicon chip.
Schoen I and Fromherz P
Journal of Neurophysiology, 100, 346-346 (2008)
Corrigendum: Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation.
Rombouts I et al.
Scientific Reports, 5 (2015)
Judith A Stolwijk et al.
The Journal of biological chemistry, 291(44), 22894-22912 (2016-10-30)
Endothelial barrier function is tightly regulated by plasma membrane receptors and is crucial for tissue fluid homeostasis; its dysfunction causes disease, including sepsis and inflammation. The ubiquitous activation of Ca2+ signaling upon phospholipase C-coupled receptor ligation leads quite naturally to
Simon A Johnston et al.
BMC cell biology, 9, 65-65 (2008-12-11)
Cells use filopodia to explore their environment and to form new adhesion contacts for motility and spreading. The Arp2/3 complex has been implicated in lamellipodial actin assembly as a major nucleator of new actin filaments in branched networks. The interplay

商品

Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

白蛋白在细胞培养中的应用

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能,并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

脂质诱导的胰岛素耐受性

脂肪组织中的胰岛素耐受性减少了循环游离脂肪酸的摄入,并增加了脂肪酶对储存甘油三酯的水解。了解与胰岛素耐受性相关的激素、细胞因子和脂肪因子。

实验方案

Primary Human Spheroid-Qualified Plateable Hepatocyte Culture Protocol

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

相关内容

Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门