Merck
All Photos(6)

A9418

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):
BSA, 牛白蛋白, 牛血清白蛋白
CAS Number:
EC 号:
MDL编号:
NACRES:
NA.75

生物来源

bovine

质量水平

产品线

BioReagent

形式

lyophilized powder

分子量

~66 kDa

technique(s)

cell culture | mammalian: suitable

缺失

≤5%

pH值(酸碱度)

5.0-5.6

溶解性

H2O: 40 mg/mL

运输

ambient

储存温度

2-8°C

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This Item
A2058A3156A4612
biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, cell culture | mammalian: suitable

pH

5.0-5.6

pH

7

pH

4.8-7.5

pH

4.8-7.5

solubility

H2O: 40 mg/mL

solubility

water: soluble (40 mg/ml)

solubility

water: soluble (5g/50ml)

solubility

H2O: soluble 40 mg/mL

一般描述

牛血清白蛋白是各种研究中的模型蛋白,由 583 个氨基酸组成。它是一种水溶性蛋白质,分子量为66.4kDa。六个α-螺旋形成了BSA的三个同源结构域。它可依赖于pH进行可逆的构象异构化。蛋白的天然结构在加热时会变得具有反应性和灵活性。

应用

牛血清白蛋白已用于流式细胞仪分析的洗涤液、组织消化液和 ELISA(酶联免疫吸附试验)的封闭液。它也被用于流式细胞仪的缓冲液重悬。

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
牛血清白蛋白被广泛用作细胞培养基,特别是无血清培养基的添加剂。它可带来一系列的好处,包括防止氧化损伤和稳定其他培养基成分,如脂肪酸和吡哆醛。

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为第五组分(Cohn fraction V);本品采用改进的Cohn氏冷乙醇分级分离法制备。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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