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Merck

A9469

Sigma-Aldrich

单克隆抗-FLAG®碱性磷酸酶 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

别名:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.32

生物源

mouse

共軛

alkaline phosphatase conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

M2, monoclonal

形狀

buffered aqueous glycerol solution

物種活性

all

濃度

~1 mg/mL

技術

indirect ELISA: 1:20,000

同型

IgG1

免疫原序列

DYKDDDDK

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... ALPL(249)

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一般說明

单克隆ANTI-FLAG® M2碱性磷酸酶是一种纯化的 IgG 1 小鼠抗体,与小牛肠碱性磷酸酶(AP)共价结合。该抗体偶联物可与FLAG®融合蛋白结合,并在融合蛋白的任何位置(N末端、Met-N末端、C末端或内部FLAG®肽)识别FLAG®表位。

應用

小鼠中产生的单克隆 ANTI-FLAG® M2-碱性磷酸酶抗体已用于:
  • 甜橙叶柄样品的直接组织印迹免疫测定
  • δ阿片受体内化的筛选
  • 采用 ELISA 筛查无细胞的蛋白表达

成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质印迹(1篇论文)

外觀

在含有50%甘油以及稳定剂和防腐剂的磷酸盐缓冲盐溶液中

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA
Layton CJ and Helling HW
Protein Science, 20(8), 1432-1438 (2011)
Waithaka Mwangi et al.
Journal of leukocyte biology, 78(2), 401-411 (2005-04-29)
Induction of immune responses against microbial antigens using DNA is an attractive strategy to mimic the immunity induced by live vaccines. Although DNA vaccines are efficacious in murine models, the requirement for multiple immunizations using high doses in outbred animals
Matthew T Doherty et al.
The Journal of biological chemistry, 287(47), 39369-39379 (2012-10-06)
Myb repeats ∼52 amino acid residues in length were first characterized in the oncogenic Myb transcription factor, which contains three tandem Myb repeats in its DNA-binding domain. Proteins of this family normally contain either one, two, or three tandem Myb
Mun Kyoung Kim et al.
Molecular and cellular biology, 30(10), 2411-2423 (2010-03-10)
The small nuclear RNA (snRNA)-activating protein complex (SNAPc) is essential for transcription of genes coding for the snRNAs (U1, U2, etc.). In Drosophila melanogaster, the heterotrimeric DmSNAPc recognizes a 21-bp DNA sequence, the proximal sequence element A (PSEA), located approximately
Beth A Rasala et al.
Plant biotechnology journal, 9(6), 674-683 (2011-05-04)
Microalgae have the potential to be a valuable biotechnological platform for the production of recombinant proteins. However, because of the complex regulatory network that tightly controls chloroplast gene expression, heterologous protein accumulation in a wild-type, photosynthetic-competent algal chloroplast remains low.

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