Merck

A9594

Sigma-Aldrich

单克隆抗-FLAG® M2-Cy3 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

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别名:
单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk
NACRES:
NA.32

生物来源

mouse

质量水平

偶联物

CY3 conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

种属反应性

all

浓度

~1 mg/mL

技术

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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A9594

单克隆抗-FLAG® M2-Cy3 小鼠抗

vibrant-m

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FLAG® M2-FITC单克隆抗体 小鼠抗

vibrant-m

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抗FLAG® BioM2单克隆抗体 小鼠抗

vibrant-m

F3040

Anti-FLAG® M1小鼠单抗 小鼠抗

conjugate

CY3 conjugate

conjugate

FITC conjugate

conjugate

biotin conjugate

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M1, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

一般描述

单克隆抗标记 M2-Cy3(小鼠IgG)抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白,并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。

应用

实现简单的一步式免疫细胞化学检测。 特别适用于检测在小鼠宿主中表达的FLAG融合蛋白,其中小鼠二抗可能引起交叉反应。

浏览我们有关其他应用的参考文献,请访问 FLAG® 文献页面。
此抗体已成功使用的应用,以及相关的同行评审论文如下。
蛋白免疫分析(Western Blotting)(1篇文章)

外形

溶于磷酸盐缓冲盐水,含有 1%BSA 和防腐剂

制备说明

用 Tris 缓冲盐水 (TBS) 稀释抗体:0.05 MTris,pH 7.4,含 0.15 M NaCl。

其他说明

免疫细胞化学的建议浓度为1-10 mg / ml。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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