跳转至内容
Merck

APF

Sigma-Aldrich

碱性磷酸酶检测试剂盒,荧光

登录查看公司和协议定价


About This Item

EC 号:
UNSPSC代码:
12352204
eCl@ss:
42010105
NACRES:
NA.53

等级

Molecular Biology

质量水平

用途

1 kit sufficient for 300 reactions (200 μl reactions)

技术

microbe id | specific enzyme detection: suitable

运输

wet ice

储存温度

−20°C

基因信息

human ... ALPL(249)

一般描述

碱性磷酸酶 (AP) 试剂盒采用荧光测定法检测 AP 活性。试剂盒能力在广泛的酶浓度范围内呈线性,这使得其特别适合用于比较分析。
碱性磷酸酶(AP)基因通常用作报告基因,用于确定启动子和增强子的强度、确定转录因子的作用、评估转染效率以及衡量分子克隆尝试成功与否。碱性磷酸酶(AP)试剂盒采用荧光法检测AP活性。检测在广泛的酶浓度范围内均呈线性,因此特别适合进行比较分析。

应用

碱性磷酸酶检测试剂盒(荧光法)可用于测定碱性磷酸酶(ALP)活性。
适用于测定碱性磷酸酶 (AP) 或分泌型碱性磷酸酶 (SEAP) 的报告基因活性。碱性磷酸酶试剂盒可用于测定启动子和增强子的强度,明确转录因子的角色,评估转染效率,衡量分子克隆尝试的成功与否。

也适用于检测多能干细胞中的 AP 活性,包括胚胎干细胞 (ESC) 和诱导多能干细胞 (iPSC)。

生化/生理作用

碱性磷酸酶(AP)有助于几种磷酸单酯的水解和磷酸转移。它可调节各种与细胞周期、凋亡、生长和信号转导通路相关的胞内过程。

特点和优势

  • 灵敏度比比色法高 100 倍
  • SEAP 可以在不破坏样品的情况下测量
  • 快速且可重现的结果
  • 安全且易于使用

组分

碱性磷酸酶试剂盒包含:
50 mL 荧光分析缓冲液 (B6558)
6 mL 稀释缓冲液 (B6433)
50μl 碱性磷酸酶控制酶,0.1 mg/mL (C9361)
2 x 1 mg 4-甲基伞形酮磷酸酯二钠 (M8168)

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Caterina Bossio et al.
Frontiers in bioengineering and biotechnology, 6, 114-114 (2018-09-14)
Optical modulation of living cells activity by light-absorbing exogenous materials is gaining increasing interest, due to the possibility both to achieve high spatial and temporal resolution with a minimally invasive and reversible technique and to avoid the need of viral
An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu 2+ bound to poly-thymine DNA
Lee JY, et al.
Royal Society of Chemistry Advances, 8(20), 11241-11246 (2018)
Darren M Brey et al.
Journal of biomedical materials research. Part A, 93(2), 807-816 (2010-03-04)
Combinatorial polymer syntheses are now being utilized to create libraries of materials with potential utility for a wide variety of biomedical applications. We recently developed a library of photopolymerizable and biodegradable poly(beta-amino ester)s (PBAEs) that possess a range of tunable
Irena Moserova et al.
PloS one, 7(3), e32972-e32972 (2012-03-10)
We have analyzed the molecular mechanisms of photoinduced cell death using porphyrins with similar structure differing only in the position of the ethylene glycol (EG) chain on the phenyl ring. Meta- and para-positioned EG chains targeted porphyrins to different subcellular
Kristen L Lee et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(3), 1157-1165 (2013-11-28)
Primary cilia are single, nonmotile, antenna-like structures extending from the apical membrane of most mammalian cells. They may mediate mechanotransduction, the conversion of external mechanical stimuli into biochemical intracellular signals. Previously we demonstrated that adenylyl cyclase 6 (AC6), a membrane-bound

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持