Merck

APOAF

Sigma-Aldrich

Annexin V-FITC 凋亡检测试剂盒

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别名:
Annexin V-FITC, Apoptosis probe FITC

用途

(20 tests)

质量水平

包装

pkg of 1 kit

technique(s)

flow cytometry: suitable

application(s)

cell analysis
detection

detection method

fluorometric

运输

wet ice

储存温度

2-8°C

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此商品
APOACCBA059S7111
usage

(20 tests)

usage

sufficient for 200 tests

usage

sufficient for 20 tests

usage

-

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

-

application(s)

-

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

一般描述

Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞,并使用流式细胞仪进行定量。AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯(FITC)偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。该试剂盒包含碘化丙啶(PI),用于标记细胞膜完全破坏的坏死细胞内的DNA。这种组合可以区分早期凋亡细胞(annexin V阳性, PI阴性),坏死细胞(annexin V阳性, PI阳性),以及活细胞(annexin V阴性, PI阴性)。

应用

Annexin V-FITC 凋亡检测试剂盒适于以下应用:
  • 在治疗前列腺癌的过程中对LNCaP前列腺癌细胞染色以测定 G. lucidum提取物活性。
  • 在研究DBP-maf(维生素D结合蛋白-巨噬细胞活化因子)基因对前列腺癌细胞活性的抑制中,用于对肿瘤细胞的标记。
  • 用于对flippase酶活性的间接测定。

特点和优势

相比基于DNA的检测法(比如TUNEL法),可检测到细胞凋亡的更早期进程。
  • 快速标记细胞。细胞染色仅需10分钟。
  • 细胞无需固定或预处理,节省检测时间并满足细胞用于进一步研究。
  • 试剂盒内含碘化丙啶二级染料,用于从活细胞和坏死细胞中区分出凋亡细胞。

其他说明

使用前使所有组分达到室温。

仅试剂盒组分

产品编号
说明

  • APOAFA

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 1


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The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
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商品

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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