AV32615

Sigma-Aldrich

Anti-HCLS1 (AB2) antibody produced in rabbit

IgG fraction of antiserum

别名:
Anti-Hematopoietic cell-specific Lyn substrate 1

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

54 kDa

species reactivity

horse, rabbit, pig, human, dog, rat, bovine

concentration

0.5 mg - 1 mg/mL

application(s)

western blot: suitable

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... HCLS1(3059)

General description

HCLS1 is a mitochondrial protein that is predominantly expressed in cardiac and skeletal tissues. This protein is known to interact with HAX1 and LEF-1 which, in turn, regulates granulocyte colony-stimulating factor (G-CSF)-mediated granulopoiesis.
Rabbit Anti-HCLS1 antibody recognizes human, rat, mouse, chicken, canine, bovine, and zebrafish HCLS1.

Immunogen

Synthetic peptide directed towards the N terminal region of human HCLS1

Application

Rabbit Anti-HCLS1 (AB2) antibody can be used for western blot applications at a concentration of 1.25μg/ml.

Biochem/physiol Actions

HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.

Sequence

Synthetic peptide located within the following region: FGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAV

Physical form

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

分析证书
原产地证书 (CofO)
Julia Skokowa et al.
Nature medicine, 18(10), 1550-1559 (2012-09-25)
We found that hematopoietic cell-specific Lyn substrate 1 (HCLS1 or HS1) is highly expressed in human myeloid cells and that stimulation with granulocyte colony-stimulating factor (G-CSF) leads to HCLS1 phosphorylation. HCLS1 binds the transcription factor lymphoid-enhancer binding factor 1 (LEF-1)...
Daohong Chen et al.
The Biochemical journal, 398(2), 169-176 (2006-05-24)
In eukaryotic cells, CLS (cardiolipin synthase) is involved in the final step of cardiolipin synthesis by catalysing the transfer of a phosphatidyl residue from CDP-DAG (diacylglycerol) to PG (phosphatidylglycerol). Despite an important role of cardiolipin in regulating mitochondrial function, a...

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