Merck

B0287

Sigma-Aldrich

单克隆抗 FITC-生物素 小鼠抗

clone FL-D6, purified immunoglobulin, buffered aqueous solution

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别名:
单克隆抗-FITC

生物来源

mouse

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

FL-D6, monoclonal

形式

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
A1812F3523C3117
Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

A1812

单克隆抗-FITC

conjugate

biotin conjugate

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

FL-D6, monoclonal

clone

FL-D6, monoclonal

clone

DI-22, monoclonal

clone

CY-96, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil

technique(s)

dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate, indirect ELISA: 1:30,000

technique(s)

dot immunobinding: 1:16 using digoxigenin-labeled biomolecule

technique(s)

direct ELISA: suitable, dot blot: suitable, immunocytochemistry: 1-2 μg/mL using chicken fibroblasts, microarray: suitable

一般描述

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特异性

该抗体可与游离的和结合的异硫氰酸荧光素(FITC)反应。

免疫原

FITC-BSA偶联物

应用

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:
  • for amplification of signal in immunofluorescence assays
  • in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
  • enzyme linked immunosorbent assay (ELISA)

生化/生理作用

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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Flow-based microimmunoassay
Hayes MA, et al.
Analytical Chemistry, 73(24), 5896-5902 (2001)
Chapter 11 - (Strept)avidin?Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Peter Stachon et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(10), 2237-2245 (2014-08-12)
Nucleotides such as ATP, ADP, UTP, and UDP serve as proinflammatory danger signals via purinergic receptors on their release to the extracellular space by activated or dying cells. UDP binds to the purinergic receptor Y6 (P2Y6) and propagates vascular inflammation
Kazuyuki Kasahara et al.
Heliyon, 8(7), e09981-e09981 (2022-07-29)
Compelling evidence suggests a crucial role for Foxp3+ regulatory T cells (Tregs) in the control of atherosclerosis. Although suppression of pro-inflammatory CD4+ T cell immune responses is supposed to be important for athero-protective action of Foxp3+ Tregs, few studies have
Maximillian A Rogers et al.
Circulation research, 121(3), 220-233 (2017-06-14)
Mitochondrial changes occur during cell differentiation and cardiovascular disease. DRP1 (dynamin-related protein 1) is a key regulator of mitochondrial fission. We hypothesized that DRP1 plays a role in cardiovascular calcification, a process involving cell differentiation and a major clinical problem

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