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一般描述
生物素被认为是一种必需的营养物质，尽管已知它是由一些肠道细菌分泌的。它微量存在于肝和脑组织中。
生物素：抗生物素蛋白第二抗体信号系统涉及生物素与第二抗体的偶联。一旦第二抗体在免疫化学测定中结合靶一抗，通过允许与酶或信号分子偶联的亲和素与抗体相关生物素分子结合来产生信号。
通过 Ouchterlony 双扩散和免疫电泳与生物素载体蛋白复合物测定特异性和反应性。未观察到与载体蛋白的反应性。
免疫原
生物素-KLH 结合物
应用
在一些应用中，生物素化探针与亲和素的定位会产生高背景水平。抗生物素试剂可替代亲和素，以减少非特异性结合。
山羊抗生物素抗体已用于多孔板试验，以鉴定脱硫生物素蛋白。此抗体还可用于色度酶免疫测定。
生化/生理作用
生物素可作为氧化代谢所需羧化酶的辅因子。摄入富含抗生物素蛋白的食物（如蛋白）会减少小肠中生物素的吸收。因此，显著降低其生物利用度，导致蛋白损伤（egg white injury）。妊娠期间通常可观察到生物素水平的下降。生物素缺乏症可能是先天的，缺陷蛋白涉及生物素稳态。
外形
从 0.01 M 磷酸钠、15 mM 氯化钠 (pH 7.2) 冻干
重悬
用 0.135 M 氯化钠复溶。
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