Merck

B4287

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
EC 号:
MDL编号:

生物来源

bovine

质量水平

等级

for molecular biology

产品线

BioReagent

检测方案

≥98%

形式

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

poly bottle of

来源

USA origin

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

pH值(酸碱度)

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

Protease free

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

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此商品
A7511A9543A2153
grade

for molecular biology

grade

-

grade

-

grade

-

assay

≥98%

assay

≥97% (agarose gel electrophoresis)

assay

≥98%

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

cold ethanol fractionation

purified by

heat shock fractionation

purified by

cold ethanol fractionation

应用

A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

特点和优势

  • Protease free
  • Suitable for blocking non-specific protein binding sites in hybridizations
  • Heat shock fractionated

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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实验方案

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

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