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B6916

Supelco

Bradford试剂

for 0.1-1.4 mg/ml protein

别名:

考马斯染料结合蛋白检测，蛋白染料试剂

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About This Item

分類程式碼代碼:
12161500
NACRES:
NA.32

品質等級

200

形狀

solution

儲存溫度

2-8°C

相关类别

一般說明

Bradford检测是把考马斯亮蓝G-250加入至蛋白溶液中。考马斯蓝染料可与碱性和芳香族氨基酸结合，从而在蛋白质测定过程中引起吸光值的迁移。

應用

Bradford试剂已用于测定总蛋白的浓度。

特點和優勢

  • 该试剂为即用型。 无需混合或稀释。
  • 显色很快速。 仅需五分钟的孵育便能将样本在595 nm处进行读值。
  • 带有硫醇的还原性糖和还原性底物并不会干扰该试剂。
  • 试剂适用于微量(1-10 μg/ml)及标准(50-1400 μg/ml)的检测。
  • 可用于微孔板检测。
  • 低成本检测。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律資訊

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象形圖

Health hazardCorrosion
GHS08,GHS05

訊號詞

Warning

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

標靶器官

Eyes,Central nervous system

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000261841
0000261841
SLCN3585
SLCK1933
SLCG1879

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Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
M Tal et al.
The Journal of biological chemistry, 260(18), 9976-9980 (1985-08-25)
Dimethyl sulfoxide was found to be effective for extraction of Coomassie Brilliant Blue R-250 (Coomassie R) from stained proteins on polyacrylamide gel slices. A good correlation was found between the ability of different proteins to bind Coomassie R and their
Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani.
Grinyer J et al.
Current Genetics, 47, 381-381 (2005)
Rosenberg IM
Protein Analysis and Purification: Benchtop Techniques (2006)
Caleigh Mandel-Brehm et al.
Neurology, 93(5), e433-e444 (2019-07-05)
To identify molecular correlates of primary angiitis of the CNS (PACNS) through proteomic analysis of CSF from a biopsy-proven patient cohort. Using mass spectrometry, we quantitatively compared the CSF proteome of patients with biopsy-proven PACNS (n = 8) to CSF
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