Merck

B6917

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

lyophilized powder, suitable for (for molecular biology), Non-acetylated

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
EC 号:
MDL编号:
NACRES:
NA.27

生物来源

bovine

等级

for molecular biology

检测方案

≥98% (agarose gel electrophoresis)

形式

lyophilized powder

质量

deacetylated

分子量

~66 kDa

纯化方式

heat shock fractionation

包装

glass bottle of

来源

USA origin

technique(s)

electrophoresis: suitable-98% using Agarose

杂质

enzymatic impurity, none detected

pH值(酸碱度)

7

溶解性

water: soluble (50 mg/ml)

适用性

suitable for (for molecular biology)

UniProt登记号

异质活性

Alkaline Phosphatase free
Alkaline phosphatase and peroxidase, none detected
DNase free
DNase, RNase, and protease, none detected
Peroxidase free
Protease free
RNase free

储存温度

2-8°C

Gene Information

bovine ... ALB(280717)

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此商品
B4287A7638A7511
grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

assay

≥98% (agarose gel electrophoresis)

assay

≥98%

assay

≥99% (agarose gel electrophoresis)

assay

≥97% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

cold ethanol and heatshock fractionation

purified by

cold ethanol fractionation

一般描述

牛血清白蛋白(BSA)是牛肝脏产生的一种血浆蛋白,具有球状、非糖基化的α-螺旋结构。它由带有583个氨基酸残基和17个半胱氨酸残基的单个多肽链组成,这些残基形成交联的二硫键,从而使结构保持稳定。BSA蛋白含有三种结构不同的结构域(I,II,III),每个结构域都含有两个心形结构的亚结构域(A和B)。

应用

牛血清白蛋白(BSA)已用于:
  • 作为磷酸盐缓冲液(PBS) 的成分用于溶解作为培养基补充剂而添加的肿瘤因子α (TNFα)
  • 作为阻滞剂用于减少白蛋白传感器分析中的非特异性结合
  • 作为免疫荧光 (IF) 测定中的阻滞剂
  • 作为布拉德福测定(Bradford assay)中的蛋白质标准品

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
牛血清白蛋白(BSA)是一种多功能蛋白,常用作细胞和组织培养基的补充剂。 它有助于溶解脂质和稳定其它蛋白质。BSA也是一种小分子(如类固醇、脂肪酸和甲状腺激素)的载体。它可用作免疫化学测试的标准蛋白,用于测定溶液的蛋白质浓度和校准研究。

特点和优势

该分子生物学级BSA产物已经过以下性能测试:
*无碱性磷酸酶
*无DNase/无核酸外切酶
*无切口酶/无核酸内切酶
*无过氧化物酶
*无蛋白酶
*无RNase

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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