Merck

B7405

Sigma-Aldrich

抗-−地高辛生物素抗体,小鼠单克隆

clone DI-22, purified from hybridoma cell culture

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别名:
单克隆抗-地高辛, 单克隆抗 -地高辛−生物素 小鼠抗
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

biotin conjugate

抗体形式

purified from hybridoma cell culture

antibody product type

primary antibodies

克隆

DI-22, monoclonal

形式

buffered aqueous solution

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule
western blot: 1:500 using using digoxin-labeled biomolecule

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
SAB4200669D8156A1054
conjugate

biotin conjugate

conjugate

-

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

clone

DI-22, monoclonal

clone

DI-22, monoclonal

clone

DI-22, monoclonal

clone

DI-22, monoclonal

form

buffered aqueous solution

form

-

form

-

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule

technique(s)

ELISA: 0.15-0.3 μg/mL

technique(s)

dot blot: suitable, flow cytometry: suitable, indirect ELISA: 1:10,000 using digoxin-BSA, indirect ELISA: 1:2,500 using digoxigenin-transferrin

technique(s)

direct ELISA: 1:10,000 using using 20 μg/ml of purified digoxin-BSA for coating and pNPP substrate, direct ELISA: 1:6,000 using using 10 μg/ml of digoxigenin-transferrin for coating and pNPP substrate, dot blot: 1:20,000 using digoxigenin-labeled biomolecules (chemiluminescent)

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一般描述

单克隆抗-地高辛(小鼠 IgG1 亚型)抗体来自于免疫小鼠骨髓瘤细胞和脾细胞相互融合而产生的杂交瘤。

特异性

抗体对地高辛具有特异性,且对地高辛具有高亲和力。

免疫原

地高辛-KLH。

应用

小鼠抗-地高辛FITC单克隆抗体可用于:
  • 原位杂交
  • 免疫组织化学
  • 免疫定位

小鼠抗地高辛生物素单克隆抗体可用于 ELISA ( 1:10,000 稀释度)和 斑点印迹(1:500 稀释度)。它还用于通过TUNEL技术对宫颈癌切片中的凋亡细胞进行染色。

生化/生理作用

地高辛是一种植物雌激素,可以与雌激素受体结合,并可在心肌中产生正性肌力。研究表明,地高辛可降低对雌激素敏感的乳腺癌和子宫癌的风险。
该偶联物可用于检测地高辛标记的化合物,例如寡核苷酸、抗体或肽。

外形

含有1% BSA及15 mM叠氮化钠的 0.01 M磷酸盐缓冲液(pH 7.4)溶液

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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