B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

别名:
5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside, Red-Gal
Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS号:
分子量:
408.63
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Quality Level

grade

for molecular biology

sterility

non-sterile

product line

BioReagent

assay

≥98.0% (HPLC)

form

powder

solubility

methanol: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1

InChI key

CHRVKCMQIZYLNM-HTOAHKCRSA-N

General description

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.

Principle

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

George Chenaux et al.
The European journal of neuroscience, 34(10), 1620-1633 (2011-11-23)
EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. As EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be...
Susan C McCutcheon et al.
BMC biology, 8, 89-89 (2010-06-24)
Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum...
Maria Belen Jaurena et al.
Nature communications, 6, 7476-7476 (2015-06-24)
All cranial placode progenitors arise from a common precursor field anterior to the neural plate, the pre-placodal region (PPR). We showed that transcription factor Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. Here...
Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and...

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