Merck

C0992

Sigma-Aldrich

Monoclonal Anti-Cy3/Cy5 小鼠抗

clone CY-96, purified from hybridoma cell culture

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别名:
Monoclonal Anti-Cy3/Cy5, Cy3 Antibody, Cy3 Antibody - Anti-Cy3/Cy5 antibody, Mouse monoclonal
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

CY-96, monoclonal

形式

buffered aqueous solution

浓度

~1.5 mg/mL

技术

direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable

同位素/亚型

IgG2a

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

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此商品
C3117A9594V6389
vibrant-m

C0992

Monoclonal Anti-Cy3/Cy5 小鼠抗

vibrant-m

A9594

单克隆抗-FLAG® M2-Cy3 小鼠抗

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

CY-96, monoclonal

clone

CY-96, monoclonal

clone

M2, monoclonal

clone

V9, monoclonal

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

CY3 conjugate

conjugate

unconjugated

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

isotype

IgG2a

isotype

IgG2a

isotype

IgG1

isotype

IgG1

一般描述

Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.

特异性

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

免疫原

mixture of proteins labeled with Cy3/Cy5.

应用

Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:
  • immunofluorescence
  • western blot
  • dot blot
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunocytochemistry
  • protein microarrays
  • In in situ hybridization

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

生化/生理作用

Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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A K Kenworthy
Methods (San Diego, Calif.), 24(3), 289-296 (2001-06-14)
Fluorescence resonance energy transfer (FRET) detects the proximity of fluorescently labeled molecules over distances >100 A. When performed in a fluorescence microscope, FRET can be used to map protein-protein interactions in vivo. We here describe a FRET microscopy method that
Wonbae Lee et al.
Nucleic acids research, 42(9), 5967-5977 (2014-03-15)
DNA constructs labeled with cyanine fluorescent dyes are important substrates for single-molecule (sm) studies of the functional activity of protein-DNA complexes. We previously studied the local DNA backbone fluctuations of replication fork and primer-template DNA constructs labeled with Cy3/Cy5 donor-acceptor
Te Liu et al.
Molecular medicine reports, 13(6), 5053-5058 (2016-04-29)
Premature ovarian failure (POF) is a common cause of female infertility, for which there are currently no ideal treatments or medications. Furthermore, apoptosis of ovarian granulosa cells (OGCs) is an important mechanism underlying the decline in ovarian reserve and function.
Transplantation of ovarian granulosa-like cells derived from human induced pluripotent stem cells for the treatment of murine premature ovarian failure
Liu T. et al.
Molecular Medicine Reports, 13(6), 5053-5058 (2016)
An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Nagai-Okatani C, et al.
International Journal of Molecular Sciences, 20(3), 700-700 (2019)

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