Merck
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C100

Sigma-Aldrich

过氧化氢酶 来源于牛肝脏

aqueous suspension, 40,000-60,000 units/mg protein (E1%/405)

别名:
H2O2:H2O2 氧化还原酶
CAS号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.54

质量水平

200

形式

aqueous suspension

specific activity

40,000-60,000 units/mg protein (E1%/405)

分子量

tetramer ~250 kDa

等电点

5.4

UniProt登记号

运输

wet ice

储存温度

2-8°C

Gene Information

cow ... CAT(280743)

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包装

1 g in serum bottle

应用

过氧化氢酶作为天然抗氧化剂,可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。 工业上,过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢,并用于检查其对DNA修复大肠杆菌突变体活力的阳性效应。

生化/生理作用

过氧化氢酶催化过氧化氢分解成水和氧气。 它还能与烷基过氧化氢反应,如甲基过氧化物和乙基过氧化物,可以用甲醇、乙醇、丙醇、甲酸盐和硝酸盐作为氢供体取代第二 H2O2 分子。

组分

牛肝脏过氧化氢酶是由4个相同亚基组成的四聚体,每个亚基的分子量为60kDa。 每个亚基都含有一个正铁血红素IX基团以及与之结合的铁。 过氧化氢酶也能紧密结合NADP,其中NADP和血红素基团的距离在13.7埃之内。

注意

过氧化氢酶溶液不可冷冻。 冷冻溶液将导致酶活性损失50-70%。

单位定义

一单位酶可在pH7.0、25℃条件下每分钟分解1.0 μM的H2O2,通过测量A240的下降速率可知H2O2的浓度从10.3降至9.2 mM。

制备说明

本品为含0.1%(w/v)百里香酚的过氧化氢酶水悬浮液,活性为40,000-60,000单位/mg蛋白质。为除去百里香酚防腐剂,可以通过离心使过氧化氢酶晶体沉淀,弃去上清液,将沉淀物重悬于水中,然后再次沉淀。该酶溶于50 mM磷酸钾缓冲液中,浓度为1 mg/mL,pH 7.0。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书

请输入批号搜索分析证书(COA)。

原产地证书 (CofO)

请输入批号搜索原产地证书(COO)。

Product Information Sheet

更多文件

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can Product C100, Catalase from bovine liver, be shipped on wet ice or dry ice?

    Product No. C100 normally ships at ambient temperature. If desired, it can be shipped on wet ice upon special request. C100 cannot be shipped on dry ice. That is, the suspension can be harmed by freezing.

  6. Is Product C100, Catalase from bovine liver, a solution or a suspension?

    Most of the protein (catalase) in Product No. C100 is a solid in suspension, with a small percentage actually in solution.

  7. Is Product C100, Catalase from bovine liver, sterile?

    C100 is not necessarily sterile. But it does contain a preservative (0.1% thymol), which should prevent microbial growth.

  8. What is the catalase concentration of Product C100, Catalase from bovine liver?

    There is no "official" specification, but the lot history shows that it has varied from 15-100 mg/mL. The concentration is provided on the certificate of analysis for each lot. Note that most of the catalase is typically a solid in suspension, with a small percentage actually in solution.

  9. Your website shows that Product No. C100 is catalase. But I have some notes that indicate that C100 is your product number for calmidazolium chloride. Which one is right?

    C-100 was used as a product number for calmidazolium chloride until about 1999 by Research Biochemicals International (RBI), which is now part of Sigma-Aldrich. Calmidazolium chloride is currently listed under Sigma Product No. C3930. So now we use the C100 only for catalase.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Shi-Ying Cai et al.
Cellular and molecular gastroenterology and hepatology, 9(4), 679-688 (2019-12-31)
Inflammation plays an important role in the pathogenesis of cholestatic liver injury, but it is unclear whether the inflammasome is involved and is the objective of this study. Gene expression was analyzed in the livers of patients with primary biliary
Thomas L Kukar et al.
Nature, 453(7197), 925-929 (2008-06-13)
Selective lowering of Abeta42 levels (the 42-residue isoform of the amyloid-beta peptide) with small-molecule gamma-secretase modulators (GSMs), such as some non-steroidal anti-inflammatory drugs, is a promising therapeutic approach for Alzheimer's disease. To identify the target of these agents we developed
Brian J McHugh et al.
PLoS pathogens, 15(4), e1007694-e1007694 (2019-04-13)
Pulmonary infections are a major global cause of morbidity, exacerbated by an increasing threat from antibiotic-resistant pathogens. In this context, therapeutic interventions aimed at protectively modulating host responses, to enhance defence against infection, take on ever greater significance. Pseudomonas aeruginosa
Ruby Peters et al.
Scientific reports, 8(1), 10418-10418 (2018-07-12)
Single molecule localization microscopy (SMLM) methods produce data in the form of a spatial point pattern (SPP) of all localized emitters. Whilst numerous tools exist to quantify molecular clustering in SPP data, the analysis of fibrous structures has remained understudied.
Romina Martínez-Rubio et al.
Physiologia plantarum, 164(1), 45-55 (2018-02-22)
Maize (Zea mays L.) suspension-cultured cells habituated to a cellulose biosynthesis inhibitor 2,6-dichlorobenzonitrile (DCB) have a modified cell wall, in which the reduction in the cellulose content is compensated by a network of highly cross-linked feruloylated arabinoxylans and the deposition

实验方案

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

相关内容

过氧化氢酶的酶学测定(EC 1.11.1.6)

本程序适用于所有过氧化氢酶产品。

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