C270

Sigma-Aldrich

单克隆抗-钙抑素 小鼠抗

clone 1F7E3D10, ascites fluid, buffered aqueous solution

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1F7E3D10, monoclonal

form

buffered aqueous solution

species reactivity

human, rat, bovine, pig

application(s)

indirect immunofluorescence: 1:200
western blot: 1:5,000

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CAST(831)
rat ... Cast(25403)

General description

钙蛋白酶抑制蛋白是人 CAST 基因编码的蛋白质。钙蛋白酶抑制蛋白是普遍存在的钙依赖性蛋白酶 μ- 和间-钙蛋白酶的内源性抑制剂。它在许多细胞生理和病理过程中起关键作用。钙蛋白酶抑制蛋白水平对于控制钙蛋白酶活性非常重要。

Specificity

表位作图研究表明,该表位位于人钙蛋白酶抑制蛋白的氨基酸 543-673(结构域 IV)之间。该抗体通过免疫印迹可以检测到 150、125、90 和 70 kDa 的条带,这些条带被认为是从钙蛋白酶抑制蛋白基因的不同起始位点翻译的蛋白质。它不会与钙蛋白酶或钙调蛋白发生交叉反应。通过免疫印迹,观察到与人血小板和红细胞、牛血小板、心脏和骨骼肌以及大鼠成肌细胞、肾脏、肝脏和脾脏的反应性。通过对猪 LLC-PK1 细胞进行免疫荧光,可以观察到弥漫性细胞质染色。

Immunogen

来自牛骨骼肌的钙蛋白酶抑制蛋白。

Application

小鼠单克隆抗-钙蛋白酶抑制蛋白抗体适用于间接免疫荧光(1:200 稀释)和蛋白质印迹(1:5,000 稀释)。

Biochem/physiol Actions

钙蛋白酶抑制蛋白在胞质中的有效性受 Ca2+ 和 cAMP 的控制。钙蛋白酶的延长活化促进钙蛋白酶抑制蛋白的降解。研究发现钙蛋白酶抑制蛋白在支原体污染的培养细胞中过表达。研究发现 CAST 基因与遗传性异质角膜营养不良症圆锥角膜(KC)的遗传易感性有关。研究还发现它与帕金森病(PD)和单核苷酸多态性(SNP)有关。

Physical form

含有 0.05% 叠氮化钠的磷酸盐缓冲盐水溶液。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

分析证书
原产地证书 (CofO)
Lan Zhang et al.
PloS one, 8(8), e70935-e70935 (2013-08-21)
Recent studies point to an association between the late-onset sporadic Parkinson's disease (PD) and single nucleotide polymorphisms (SNPs) rs1559085 and rs27852 in Ca(2+)-dependent protease calpain inhibitor calpastatin (CAST) gene. This finding is of interest since loss of CAST activity could...
Manting Du et al.
Food chemistry, 274, 743-749 (2018-10-31)
The objective of this study was to investigate the effect of phosphorylation on the sensitivity of μ-calpain to the inhibition induced by calpastatin. Purified μ-calpain was incubated with alkaline phosphatase (AP) or protein kinase A (PKA) to modulate the phosphorylation...
H Persson et al.
Brain research, 611(2), 272-278 (1993-05-21)
The localization of the two Ca-activated extralysosomal proteases m-calpain and mu-calpain in the eye of the adult rabbit was examined by immunohistochemistry, using poly- and monoclonal antibodies against the corresponding rabbit antigens. Immunoreactivity against the two forms of calpains was...
H Ma et al.
Journal of biochemistry, 113(5), 591-599 (1993-05-01)
Calpain requires Ca2+ for both proteolysis of its substrates and interaction with its endogenous inhibitor, calpastatin. The mechanism of inhibition of calpain by calpastatin has remained unsolved, although Nishimura and Goll [J. Biol. Chem. 266, 11842-11850 (1991)] reported that autolyzed...
M Averna et al.
Cellular and molecular life sciences : CMLS, 60(12), 2669-2678 (2003-12-20)
The amount of calpastatin directly available in cytosol is under the control of [Ca2+] and [cyclic AMP]. Prolonged calpain activation also promotes degradation of calpastatin. The fluctuation of calpastatin concentration in cell soluble fraction is accompanied by an initial decrease...

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