Merck

C3117

Sigma-Aldrich

Monoclonal Anti-Cy3/Cy5−Biotin antibody produced in mouse

~1 mg/mL, clone CY-96, purified immunoglobulin, buffered aqueous solution

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别名:
Monoclonal Anti-Cy3/Cy5
NACRES:
NA.46

生物来源

mouse

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

CY-96, monoclonal

形式

buffered aqueous solution

浓度

~1 mg/mL

technique(s)

direct ELISA: suitable
dot blot: suitable
immunocytochemistry: 1-2 μg/mL using chicken fibroblasts
microarray: suitable

同位素/亚型

IgG2a

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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小鼠抗Cy5单克隆抗体

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

biotin conjugate

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

CY-96, monoclonal

clone

CY5-15, monoclonal

clone

DI-22, monoclonal

clone

FL-D6, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

concentration

~1 mg/mL

concentration

~1.5 mg/mL

concentration

-

concentration

-

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一般描述

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

特异性

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

免疫原

mixture of proteins labeled with Cy3/Cy5.

应用

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • dot blot assay
  • protein microarrays
  • immunofluorescenc

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

生化/生理作用

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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