所有图片(1)

C3228

Sigma-Aldrich

细胞裂解 MT细胞裂解试剂

For mammalian tissues

NACRES:
NA.56

质量水平

200

应用

细胞分解MT细胞裂解试剂已用于海马组织、小鼠四头肌、原代人滋养层(PHT)、小鼠比目鱼肌和柯蒂氏器官(OC)的均质化。

特点和优势

  • 温和:非变性,不会干扰下游应用
  • 便捷:提供即用型

其他说明

细胞分解MT是提取蛋白质的有效试剂。裂解缓冲液由一种可透析的温和洗涤剂、二甲胺和150 mM氯化钠组成,对蛋白质相互作用和生物活性的干扰最小。细胞分解MT也用于提取细胞系蛋白质。20 mL细胞分解MT的体积足以容纳1克组织。

质量

已进行测试,但不限于以下组织:大鼠大脑、肾脏、肌肉、心脏、肝脏、脾脏和小鼠大脑、肾脏和肌肉。

法律信息

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Pasireotide protects mammalian cochlear hair cells from gentamicin ototoxicity by activating the PI3KAkt pathway
Kucharava K, et al.
Cell death & disease, 10(2), 110-110 (2019)
Mechanistic Target of Rapamycin Complex 1 Promotes the Expression of Genes Encoding Electron Transport Chain Proteins and Stimulates Oxidative Phosphorylation in Primary Human Trophoblast Cells by Regulating Mitochondrial Biogenesis
Rosario FJ, et al.
Scientific Reports, 9(1), 246-246 (2019)
Joseph S Marino et al.
The Journal of biological chemistry, 291(50), 25776-25788 (2016-10-28)
Glucocorticoids (GCs) regulate energy supply in response to stress by increasing hepatic gluconeogenesis during fasting. Long-term GC treatment induces hepatic steatosis and weight gain. GC signaling is coordinated via the GC receptor (GR) GRα, as the GRβ isoform lacks a...
Aerobic exercise, but not metformin, prevents reduction of muscular performance by AMPk activation in mice on doxorubicin chemotherapy
de Lima EA, et al.
Journal of Cellular Physiology, 233(12), 96529662-96529662 (2018)
Quantitative expression and localization of GABAB receptor protein subunits in hippocampi from patients with refractory temporal lobe epilepsy
Sheilabi MA, et al.
Neuropharmacology, 136, 117128-117128 (2018)

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