Merck
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C3515

Sigma-Aldrich

过氧化氢酶 来源于黑曲霉

ammonium sulfate suspension, ≥4,000 units/mg protein

别名:
H2O2:H2O2 氧化还原酶
CAS号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.54

质量水平

200

形式

ammonium sulfate suspension

specific activity

≥4,000 units/mg protein

分子量

tetramer ~250 kDa

运输

wet ice

储存温度

2-8°C

应用

艰难梭菌孢子外壳蛋白的功能鉴定中,使用 Sigma 过氧化氢酶作为阳性对照。

包装

10, 25 mg in glass bottle

生化/生理作用

过氧化氢酶催化过氧化氢分解为水和氧气。四聚体的每个亚基都含有与铁结合的正铁血红素IX基团。该酶还可强烈结合NADP,NADP紧邻血红素基团。测得等电点为 6.5。催化活性的最适 pH 为 7.0。酶活性受3-氨基-1-H-1,2,4-三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、联茴香胺和硝酸盐的抑制。将过氧化氢酶与抗坏血酸盐或抗坏血酸盐/Cu2+进行孵育,会导致过氧化氢酶分子的降解。

注意

不建议冷冻溶液。

单位定义

一单位酶可在pH7.0、25℃下每分钟分解1.0 μmol的H2O2,通过测量A240的下降速率可知H2O2浓度从10.3降至9.2 mM。

外形

在 3.2 M (NH 4 ) 2 SO 4 溶液 (pH 6.0) 中的混悬液

分析说明

双缩脲法测蛋白

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

请输入批号搜索分析证书(COA)。

原产地证书 (CofO)

请输入批号搜索原产地证书(COO)。

更多文件

Quotes and Ordering

Jacek Switala et al.
Archives of biochemistry and biophysics, 401(2), 145-154 (2002-06-11)
Catalases from 16 different organisms including representatives from all three phylogenetic clades were purified and characterized to provide a comparative picture of their respective properties. Collectively the enzymes presented a diverse range of activities and properties. Specific activities ranged from
Alejo Mosqueira et al.
Journal of neurochemistry, 152(6), 663-674 (2019-11-30)
Synaptic strength depends on the number of cell-surface neurotransmitter receptors in dynamic equilibrium with intracellular pools. Dysregulation of this homeostatic balance occurs, for example in myasthenia gravis, an autoimmune disease characterized by a decrease in the number of postsynaptic nicotinic
Patima Permpoonpattana et al.
Journal of bacteriology, 195(7), 1492-1503 (2013-01-22)
Spores of Clostridium difficile play a key role in the dissemination of this important human pathogen, and until recently little has been known of their functional characteristics. Genes encoding six spore coat proteins (cotA, cotB, cotCB, cotD, cotE, and sodA)
Nicolas Olivier et al.
PloS one, 8(7), e69004-e69004 (2013-07-23)
Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolutions down to a few tens of nanometers by exploiting the cycling of
Lydia Farack et al.
STAR protocols, 1(1), 100007-100007 (2020-10-29)
We describe an optimized smFISH protocol for the intact pancreas. The protocol is adapted from Lyubimova et al. (2013), a generic tissue smFISH protocol that works for most tissues but not the pancreas. The main changes implemented include increasing the period

技术文章

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

实验方案

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

相关内容

过氧化氢酶的酶学测定(EC 1.11.1.6)

本程序适用于所有过氧化氢酶产品。

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以硫酸铵悬浮液形式提供的酶的使用指南

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