C4129

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

CAS号:
EC 号:
MDL编号:
eCl@ss:
42010112

质量水平

300

类型

Type II

形式

lyophilized powder

specific activity

≥40 units/mg protein

分子量

25 kDa

组成

protein, ≥85%

颜色

white to off-white

特色行业

Diagnostic Assay Manufacturing

UniProt登记号

储存温度

−20°C

Gene Information

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相关类别

应用

sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系,使用固定化金属离子亲和层析(IMAC)和固定化金属离子亲和毛细管电泳(IMACE)。

包装

250, 500 mg in poly bottle
1, 10 g in poly bottle

生化/生理作用

α-胰凝乳蛋白酶是丝氨酸肽酶,并且具有通过二硫键连接的三条多肽链(A 链-13 残基,B 链-131 残基和 C 链-97 残基)中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca2+ 激活和稳定酶。该酶被二异丙基氟磷酸盐(DFP)、甲苯磺酰氟(PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK)、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu2+ 和 Hg2+ 抑制。
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。

单位定义

一个单元将在 pH 7.8,25℃下,每分钟水解 1.0 μmole BTEE。

制备说明

以 3×结晶的胰凝乳蛋白酶原为原料生产

分析说明

通过 E1%/280 确定的蛋白质

其他说明

警示用语:

Danger

hazcat

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of alpha-chymotrypsin from bovine pancreas, product C4129?

    The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.

  6. How do you recommend preparing a solution of Product No. C4129?

    It is recommended to reconstitute Product No. C4129 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as a stabilizer, and possibly an activator, of the enzyme.

  7. How can solutions of alpha chymotrypsin (Product No. C4129) be stored?

    Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.

  8. What is the pH optimum for the enzyme, Product No. C4129?

    The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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K Y Jiang et al.
Biochimica et biophysica acta, 1433(1-2), 198-209 (1999-08-14)
Chemical glycosylation of bovine alpha-chymotrypsin, by a glucosamine adduct on the carboxyl group, results in the modification of its catalytic activity. The structural alterations of alpha-chymotrypsin resulting from its glycosylation are studied by immobilized metal-ion affinity chromatography (IMAC) and immobilized...
Gang Huang et al.
Nature communications, 8(1), 935-935 (2017-10-19)
Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and long read-length DNA sequencing applications. The analysis and sequencing of proteins, however, is complicated by their folded structure and non-uniform charge. Here we show that an electro-osmotic flow through Fragaceatoxin...
技术文章
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
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实验方案
Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.
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相关内容
按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。
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