Merck

C4129

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

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别名:
α-chymotrypsin A and B, alpha-chymotrypsin
CAS号:
EC 号:
MDL编号:
eCl@ss:
42010112

类型

Type II

质量水平

形式

lyophilized powder

比活

≥40 units/mg protein

分子量

25 kDa

组成

protein, ≥85%

颜色

white to off-white

UniProt登记号

应用

diagnostic assay manufacturing

储存温度

−20°C

基因信息

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此商品
CHY5S1134007C2160000
α-糜蛋白酶 来源于牛胰腺 Type II, lyophilized powder, ≥40 units/mg protein

C4129

α-糜蛋白酶 来源于牛胰腺

α-糜蛋白酶 来源于牛胰腺 ≥40 units/mg protein, vial of 5 mg

CHY5S

α-糜蛋白酶 来源于牛胰腺

USP

1134007

糜蛋白酶

vibrant-m

C2160000

糜蛋白酶

specific activity

≥40 units/mg protein

specific activity

≥40 units/mg protein

specific activity

-

specific activity

-

form

lyophilized powder

form

solid

form

-

form

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

UniProt accession no.

P00767

UniProt accession no.

P00767

UniProt accession no.

-

UniProt accession no.

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

storage temp.

2-8°C

应用

sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系,使用固定化金属离子亲和层析(IMAC)和固定化金属离子亲和毛细管电泳(IMACE)。

生化/生理作用

α-胰凝乳蛋白酶是丝氨酸肽酶,并且具有通过二硫键连接的三条多肽链(A 链-13 残基,B 链-131 残基和 C 链-97 残基)中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca2+ 激活和稳定酶。该酶被二异丙基氟磷酸盐(DFP)、甲苯磺酰氟(PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK)、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu2+ 和 Hg2+ 抑制。
一种丝氨酸蛋白酶,能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。

单位定义

一个单元将在 pH 7.8,25℃下,每分钟水解 1.0 μmole BTEE。

制备说明

以 3×结晶的胰凝乳蛋白酶原为原料生产

分析说明

通过 E1%/280 确定的蛋白质

其他说明

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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商品

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

按照我们的程序进行胰凝乳蛋白酶活性测定。该α-胰凝乳蛋白酶测定将会指导您完成整个过程以及必要的计算。

Chromatograms

application for HPLC

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