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Merck

C4356

Sigma-Aldrich

Anti-Caspase 9 antibody,Mouse monoclonal

clone CAS9, purified from hybridoma cell culture

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

CAS9, monoclonal

形狀

buffered aqueous solution

分子量

antigen 37 kDa

物種活性

human

濃度

~2 mg/mL

技術

indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a purified recombinant human caspase 9 preparation

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... CASP9(842)

一般說明

Monoclonal Anti-Caspase 9 (mouse IgG1 isotype) is derived from the CAS9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to amino acids of human caspase 9 protein, conjugated to keyhole limpet hemocyanin (KLH). Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspases are normally present in the cell as inactive procaspases. Caspase 9 (also known as Mch6 and ICE-LAP6) gene encodes a protein product of 37 kDa (reported also as 46 kDa), which undergoes a cleavage upon activation, leading to p18/p15/p9 subunits. It is a member of the CED-3 subfamily, bearing high similarity to caspase. High level expression of caspase 9 is found in the heart, testis and ovary.

免疫原

synthetic peptide corresponding to amino acids 299-316 of human caspase 9 protein conjugated to KLH.

應用

Anti-Caspase 9 antibody,Mouse monoclonal has been used in:
  • western blot assay
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA).

生化/生理作用

Caspase 9 is a downstream protease, activated in caspase 3- and granzyme B-mediated apoptosis. Procaspase 9 is activated when a complex is formed with the cofactor apoptotic protease activating factor-1 (Apaf-1) through the caspase recruitment domain (CARD). Caspase 9 plays a critical role in the survival of neuronal progenitors within the embryonic forebrain and cortex. Its deficiency leads to defective brain development and embryonic lethality. Overexpression of caspase 9, but not of a mutant in which the catalytic Cys was replaced with an Ala, induces apoptosis in MCF7 cells.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Role of caspases in the regulation of apoptotic pancreatic islet beta-cells death
Hui H, et al.
Journal of Cellular Physiology, 200(2), 177-200 (2004)
Apoptosis dependent and independent functions of caspases
Madame Curie Bioscience Database [Internet]. (2013)
Generation of Advanced Glycation End-Products (AGEs) by glycoxidation mediated by copper and ROS in a human serum albumin (HSA) model peptide: reaction mechanism and damage in motor neuron cells
Marques CMS, et al.
Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 824(6), 42-51 (2017)
CD 47 blockade reduces ischemia/reperfusion injury in donation after cardiac death rat kidney transplantation
Wang X, et al.
American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 18(4), 843-854 (2018)
Neural-specific inactivation of ShcA results in increased embryonic neural progenitor apoptosis and microencephaly
McFarland KN, et al.
The Journal of Neuroscience, 26(30), 7885-7897 (2006)

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