Merck

C5585

Sigma-Aldrich

单克隆抗-生物素

clone BN-34, purified immunoglobulin, buffered aqueous solution

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别名:
Monoclonal Anti-Biotin
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

CY3 conjugate

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

BN-34, monoclonal

形式

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
indirect immunofluorescence: 1:200

同位素/亚型

IgG1

运输

wet ice

储存温度

2-8°C

target post-translational modification

unmodified

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此商品
C3117F4024A1559
Monoclonal Anti-Biotin–Cy3™ antibody produced in mouse clone BN-34, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

C5585

单克隆抗-生物素

Sigma-Aldrich

Sigma-Aldrich

A1559

单克隆抗-生物素

conjugate

CY3 conjugate

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

BN-34, monoclonal

clone

CY-96, monoclonal

clone

BN-34, monoclonal

clone

BN-34, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

PBS suspension

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, indirect immunofluorescence: 1:200

technique(s)

direct ELISA: suitable, dot blot: suitable, immunocytochemistry: 1-2 μg/mL using chicken fibroblasts, microarray: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

一般描述

该抗体可使用竞争性ELISA识别游离的生物素和生物胞素。通过使用间接ELISA或免疫组织化学染色,该抗体还可识别与各种免疫球蛋白耦合的生物素。特异性是通过使用生物素化山羊抗体与涂覆在多孔板上的人和兔抗原而评估的。
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is a naturally occurring vitamin and co-enzyme required by cells in living organisms or in culture.

免疫原

生物素-KLH偶联物

应用

该抗体可被用于扩增技术、ELISA、免疫印迹、原位核酸杂交、流式细胞术、图像分析或共聚焦显微镜。
在一些应用中,生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。
Monoclonal Anti-Biotin−Cy3 antibody produced in mouse has been used in:
  • fluorescent microscopy
  • RNA fluorescence in situ hybridization (FISH){36
  • immunostaining

生化/生理作用

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Monoclonal Anti-Biotin- Cy3 can be used in many other applications where biotin can be introduced as target label. It can be used in detection of DNA and mRNA by sensitive non-isotopic in situ hybridization. It may also be used for detection of microinjected biotin-haptenized cytoskeletal proteins allowing examination of the pattern of incorporation and turnover of cytoskeletal proteins in living cells.

外形

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

法律信息

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Jennifer A Ralph et al.
The American journal of pathology, 170(3), 1121-1133 (2007-02-27)
Peripheral corticotropin-releasing hormone (CRH) is an important regulator of localized inflammatory responses. The aim of this study is to define the pathological signaling pathways in which peripheral CRH receptor-mediated responses reside. We report that PECAM-1-expressing synovial membrane endothelial cells are
Cyril Charbonnel et al.
Plant physiology, 177(1), 311-327 (2018-04-07)
Despite intensive searches, few proteins involved in telomere homeostasis have been identified in plants. Here, we used pull-down assays to identify potential telomeric interactors in the model plant species Arabidopsis (Arabidopsis thaliana). We identified the candidate protein GH1-HMGA1 (also known
High-mobility group nucleosome-binding domain 2 protein inhibits the invasion of Klebsiella pneumoniae into mouse lungs in vivo
Zheng S, et al.
Molecular Medicine Reports, 12(1), 1279-1285 (2015)
Qun Fang et al.
Medical science monitor : international medical journal of experimental and clinical research, 22, 3506-3513 (2016-10-04)
BACKGROUND In this study, we aimed to investigate the association between UCA1 and miR-27b in gastric cancer and further study their involvement in multi-drug resistance (MDR) of gastric cancer. MATERIAL AND METHODS The microarray data of dysregulated lncRNAs in gastric
Surface plasmon excitation in semitransparent inverted polymer photovoltaic devices and their applications as label-free optical sensors
Park B, et al.
Light: Science & Applications, 3(12), e222-e222 (2014)

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