Merck
所有图片(3)

C5670

Sigma-Aldrich

氯化钙

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

线性分子式:
CaCl2
CAS号:
分子量:
110.98
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.71

等级

anhydrous

蒸汽压

0.01 mmHg ( 20 °C)

产品线

BioReagent

测定

≥96.0%

形式

powder

technique(s)

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

SMILES string

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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一般描述

氯化钙是一种常用的生化试剂。 钙在许多生物过程中起重要作用,如信号传导、肌肉收缩以及细胞膜和细胞壁的稳定性维持。1,2关于生物钙的实验测量已有大量文献发表。3,4氯化钙可用于感受态大肠杆菌的制备和转化,以及真核细胞的质粒DNA或高分子量基因组DNA转染。5已有研究显示质粒DNA pBR322可通过 CaCl2介导的电穿孔法导入大肠杆菌。6已有发表文献采用通过CaCl2浓缩病毒载体的实验方案。7CaCl2已用于大肠杆菌来源的NADH-泛醌氧化还原酶(复合体I)的稳定和二维结晶。8在CaCl2和柠檬酸钠存在下结晶的猪胰弹性蛋白酶显示其金属结合位点结合的是钙离子。9已通过多孔荧光法测定CaCl2对大肠杆菌微管蛋白样FtsZ分裂蛋白聚合化时总荧光强度的影响。10

包装

100, 500 g in poly bottle

应用

该产品已经过昆虫细胞培养测试(0.5 mg/L),证明其适用于昆虫细胞培养。氯化钙可用于感受态大肠杆菌的制备和转化,
以及真核细胞的质粒DNA或高分子量基因组DNA转染。已有研究显示质粒DNA pBR322可通过CaCl2介导的电穿孔法导入大肠杆菌。已有发表文献采用通过CaCl2浓缩病毒载体的实验方案。

制备说明

该产品可溶于水(294 mg/ml),形成澄清无色溶液。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

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原产地证书 (CofO)

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更多文件

Quotes and Ordering

J Philippe et al.
International journal of obesity (2005), 39(2), 295-302 (2014-06-04)
A significant proportion of severe familial forms of obesity remain genetically elusive. Taking advantage of our unique cohort of multigenerational obese families, we aimed to assess the contribution of rare mutations in 29 common obesity-associated genes to familial obesity, and
Michael A Goren et al.
Nature communications, 5, 5115-5115 (2014-10-09)
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of
Mohsen Akbari et al.
Advanced functional materials, 24(26), 4060-4067 (2014-11-21)
The fabrication of cell-laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
Gemma L Carvill et al.
Neurology, 82(14), 1245-1253 (2014-03-14)
To determine the genes underlying Dravet syndrome in patients who do not have an SCN1A mutation on routine testing. We performed whole-exome sequencing in 13 SCN1A-negative patients with Dravet syndrome and targeted resequencing in 67 additional patients to identify new

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