所有图片(1)

C5914

Sigma-Aldrich

非酶细胞裂解液 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

MDL编号:
NACRES:
NA.75

质量水平

200

无菌性

sterile-filtered

产品线

BioReagent

形式

solution

浓度

1 ×

应用

cell culture | mammalian: suitable

pH值(酸碱度)

6.5-7.5

运输

wet ice

储存温度

2-8°C

正在寻找类似产品? Visit 产品对比指南

一般描述

在人间充质细胞中通过非酶方法进行的细胞分离有助于脱离并保持其功能,特别是细胞表面趋化因子受体的表达。

应用

细胞解离液非酶1x已用于视网膜星形胶质细胞培养基、 U87胶质母细胞瘤肿瘤细胞和心肌干细胞(CSC)的解离。
特殊的非酶配方,用于从塑料或玻璃表面温和地剥离贴壁细胞。

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

  6. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    Yes, it does contain EDTA; the concentration is proprietary.

  7. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    It will work with most cell types.

  8. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential....
Immunoimaging of CXCR4 expression in brain tumor xenografts using SPECT/CT
Nimmagadda S, et al.
Journal of Nuclear Medicine, 50(7), 1124-1130 (2009)
Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门