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C6137

灰链霉菌来源几丁质酶

lyophilized powder (essentially salt free), ≥200 units/g solid

别名:

N-acetyl-glucosaminidasechitobiase, poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, Chitin digestion enzymes

EC 号:

232-578-7

质量水平

300

形式

lyophilized powder (essentially salt free)

specific activity

≥200 units/g solid

分子量

30 kDa

溶解性

H₂O: soluble 0.90-1.10 mg/mL

储存温度

−20°C

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一般描述

几丁质酶是降解几丁质的酶的细胞外复合物。几丁质是真菌的细胞壁成分，是多种生物的外骨骼必需品，可重塑自身的几丁质或消化/溶解其他生物的甲壳质（昆虫、真菌、酵母和藻类以及其他脊椎动物的内部结构）。在许多微生物和植物中检测到几丁质酶。在真菌中，几丁质酶有助于形态发育，从而破坏真菌细胞壁固有的几丁质含量。植物几丁质酶通过相同的真菌细胞壁靶向来抵抗真菌侵袭并抑制真菌的生长。在细菌中，细菌几丁质酶有助于利用几丁质作为碳源和能源。灰链霉菌如果以几丁质为碳完成生长诱导，会产生多个不同分子量的几丁质酶。

几丁质酶水解为N-乙酰基-D-葡萄糖胺涉及两个连续的酶反应：

第一个反应是壳聚糖糊精-几丁质酶，其是一种聚（β-(1→4)-[2-乙酰氨基-2-脱氧-D-葡萄糖苷]）-糖基水解酶。可以去除几丁质的壳二糖基团。
第二个活性位是N-乙酰基-氨基葡萄糖苷酶壳二糖酶，该酶将二糖裂解为单体亚基N-乙酰基-D-葡萄糖胺。

应用

农业领域：病原体控制。
人类医疗：哮喘。
制药：壳寡糖和N-乙酰D氨基葡萄糖的制备，
单细胞蛋白的制备
从真菌和酵母中分离原生质体
致病真菌控制
处理几丁质废弃物、灭蚊和形态发育

包装

5, 25, 50 units in glass bottle

生化/生理作用

几丁质酶是一种细胞外酶复合物，可降解几丁质，分子量约为30 kDa。几丁质经过2个酶促反应，被降解为N-乙酰基-D-葡糖胺。首先，壳二糖酶-几丁质酶去除壳多糖中的壳二糖单元。在第二个反应中，N-乙酰基-氨基葡糖苷酶-壳二糖酶将二糖切割成其单体亚基（由N-乙酰基-D-葡糖胺组成）。最佳反应温度为37°C。

特点和优势

几丁质酶是降解几丁质的酶的细胞外复合物。它是一种适用于真菌细胞壁裂解的裂解酶。

单位定义

一个酶活性单位是指在2小时测定中，在pH6.0、25℃条件下每小时分解几丁质释放1.0mg N-乙酰基-D-葡糖胺所需的酶量。
一个新1小时单位=约50个48小时单位。

象形图

GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

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原产地证书 (CofO)

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Shigeo Suzuki et al.

International journal of biological macromolecules, 43(1), 13-19 (2007-11-06)

Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R

Identification of S-RNase and peroxidase in petunia nectar.

Melissa S Hillwig et al.

Journal of plant physiology, 168(7), 734-738 (2010-11-26)

Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To

An endophytic Fusarium-legume association is partially dependent on the common symbiotic signalling pathway.

Vasiliki Skiada et al.

The New phytologist, 226(5), 1429-1444 (2020-01-31)

Legumes interact with a wide range of microbes in their root systems, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so-called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in

Chitin Extraction and Content Measurement in Magnaporthe oryzae.

Xinyu Liu et al.

Bio-protocol, 7(5), e2164-e2164 (2017-03-05)

Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as

Chitinase-resistant hydrophilic symbiotic factors secreted by Frankia activate both Ca(2+) spiking and NIN gene expression in the actinorhizal plant Casuarina glauca.

Chabaud, et al.

The New phytologist, 209, 86-93 (2020)

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