Merck
  • C7611
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C7611

Sigma-Aldrich

抗-半胱天冬酶12单克隆抗体 大鼠抗

clone 14F7, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-CASP-12, Anti-CASP12P1
MDL编号:
NACRES:
NA.41

生物来源

rat

偶联物

unconjugated

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

14F7, monoclonal

形式

buffered aqueous solution

分子量

antigen ~50 kDa

species reactivity

rat, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole cell extract of rat kidney cells NRK cells

同位素/亚型

IgG2a

UniProt登记号

运输

dry ice

储存温度

−20°C

Gene Information

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C1104C5737WH0023581M1
biological source

rat

biological source

rat

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

14F7, monoclonal

clone

11E4, monoclonal

clone

84803.111, monoclonal

clone

4C9, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

mol wt

antigen ~50 kDa

mol wt

antigen (full length) 35 kDa, antigen (subunit) 20 kDa

mol wt

-

mol wt

-

一般描述

单克隆抗胱天蛋白酶12(大鼠IgG2a亚型)来自14F7杂交瘤,该杂交瘤是通过将小鼠骨髓瘤细胞与用重组小鼠胱天蛋白酶12免疫的CD大鼠的脾细胞融合而产生的。胱天蛋白酶(需要半胱氨酸的门冬氨酸蛋白酶)是一类蛋白酶,在氨基酸序列,结构和底物特异性方面具有相似性。胱天蛋白酶12在小鼠组织中普遍表达,在肌肉,肝脏和肾脏中以高水平表达,在大脑中以中等水平表达,在皮质神经元,浦肯野细胞,脑干神经元和嗅觉神经元中存在。
胱天蛋白酶12是一种蛋白,属于称为胱天蛋白酶的酶家族,可在C末端门冬氨酸残基处裂解其底物。人胱天蛋白酶12基因位于11q22号染色体上胱天蛋白酶 1/ICE基因簇内的单个基因座。功能在人类中丧失,它们不能在阿尔茨海默′病中发挥作用。胱天蛋白酶-12在内质网(ER)中检测到,在ER介导的凋亡和炎性胱天蛋白酶激活途径中起作用。

特异性

识别全长和可能截短的p20胱天蛋白酶12。

免疫原

重组小鼠胱天蛋白酶12(残基95-318)。

应用

单克隆抗胱天蛋白酶12已用于免疫印迹和免疫细胞化学(4%多聚甲醛固定,石蜡包埋)。

生化/生理作用

胱天蛋白酶 12(CASP12)在感染期间具有下调的功能,并且可能在预防炎性疾病中起作用。它与非裔美国人的类风湿性关节炎有关。胱天蛋白酶-12抑制胱天蛋白酶-1的活化,并与细菌性脓毒症的易感因子有关,但不影响对念珠菌性脓毒症的易感性。
鼠胱天蛋白酶12(CASP12)在内质网应激过程中被激活,并激活作用于底物的其他胱天蛋白酶,最终导致凋亡。在动物模型中,已证明蛋白在视网膜变性过程中被激活。在小鼠模型中,CASP12已显示可引起缺血/再灌注引起的神经元死亡。Casp12可能通过介导κB抑制剂(IκBα)的翻译后降解来刺激核因子-κB(NF-κB)。胱天蛋白酶12的过表达诱导凋亡,其被泛胱天蛋白酶抑制剂苄氧羰基-缬氨酰-丙氨酰-门冬氨酰-[O-甲基]-氟甲基酮(z-VAD-FMK)抑制。

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

制备说明

从生物反应器中的杂交瘤细胞培养物上清液中纯化获得。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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