C7923

Sigma-Aldrich

Monoclonal Anti-CD44 antibody produced in mouse

clone A3D8, purified immunoglobulin, buffered aqueous solution

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A3D8, monoclonal

form

buffered aqueous solution

mol wt

antigen 80-95 kDa

species reactivity

human

application(s)

flow cytometry: 5 μL using 1 × 106 cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... CD44(960)

General description

CD44 is a glycosylated cartilage-linked protein. The protein exists in a variety of forms with different molecular structures, ranging from 85 kDa to 250 kDa. It is a multistructural and multifunctional cell surface molecule and involved in various biological processes such as cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. Although all the activities are related to normal cell, but it has also some pathologic activities of cancer cells.

Specificity

Recognizes the CD44 (80-95 kDa) human cell surface glycoprotein on B and T lymphocytes, monocytes, granulocytes and red blood cells. The epitope recognized by this antibody is sensitive to bromelain and other proteolytic enzymes. It is detectable in routine formalin-fixed, paraffin-embedded tissue.
5th Workshop: code no. BP406, S320

Immunogen

circulating malignant human Sezary T cells.

Application

Monoclonal Anti-CD44 antibody was used as a hyaluronic acid receptor for indirect immunoperoxidase technique in a study to evaluate the relationship of lymphoid cells and macrophages with vasculature and stromal components.
Anti CD44 monoclonal antibody was used for:
  • immunostaining of nonpapillary carcinoma fine-needle aspiration (FNA) or surgical excision specimen.
  • flow cytometry analysis to assess breast CSCs in human MCF-7/Dox breast cancer cells.
  • staining of CD44 in immunohistochemistry analysis.
  • incubation of osteoclasts (OCs) in a study to analyze the molecular composition and F-actin organization of the OC podosome belts.
  • It is also suitable for western blot at a dilution of 1:500-1:2,000
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Biochem/physiol Actions

The CD44 antigen is expressed on a variety of cell types including peripheral blood leukocytes (B and T lymphocytes, monocytes, granuloctes) and red cells. It is also weakly expressed on platelets. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells. The epitope recognized by this clone is sensitive to formalin fixation and paraffin embedding.

Target description

The CD44 antigen has been studied under the following names: Pgp-1, Hermes antigen, ECM-III, H-CAM and HUTCH-1. It functions as a homing receptor. CD44 antigen is the cellular receptor for hyaluronic acid. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

Store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

2

S M Albelda
Laboratory investigation; a journal of technical methods and pathology, 68(1), 4-17 (1993-01-01)
Despite rapid advances in our understanding of the biology of cell adhesion, the data available in the literature make it is difficult to propose one simple scheme in which cell adhesion molecules can be related to tumor growth and metastasis....
Ping Chen et al.
Molecular carcinogenesis, 54(12), 1678-1685 (2014-11-20)
The anti-CD44 monoclonal antibody (mAb) A3D8 induces differentiation or apoptosis in vitro in various subtypes of acute myeloid leukemia (AML) via p27(Kip1) upregulation. Bone marrow (BM) stromal cells play a vital role in the development of chemoresistance in AML cells...
B R Zetter
Seminars in cancer biology, 4(4), 219-229 (1993-08-01)
It is now clear that adhesive interactions play a critical role in the process of metastatic tumor dissemination. Adhesion molecules act as both positive and negative modulators of the metastatic process. Molecules such as E-cadherin that promote homotypic tumor cell...
David Naor et al.
Critical reviews in clinical laboratory sciences, 39(6), 527-579 (2002-12-18)
CD44 is a multistructural and multifunctional cell surface molecule involved in cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as...
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